Expression patterns of ethylene and polyamine biosynthetic genes during fruit ripening in strawberry
April 11, 2019
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| Article Title: | Expression patterns of ethylene and polyamine biosynthetic genes during fruit ripening in strawberry |
| Authors: | Mendel. Á., Kovács L., Szentgyögyi A., Fekete S., Posta K., Kiss E. |
| Affiliation: | 1 Research Institute for Fruitgrowing and Ornamentals, National Agricultural Research and Innovation Center, Cegléd, Hungary 2 Institute of Genetics, Microbiology and Biotechnology, Szent István University, Gödöllő, Hungary |
| Abstract: | The expression pattern of genes related to ethylene and polyamine biosynthesis were analyzed in octoploid strawberry fruits in different ripening phases. RNA was isolated from Fragaria x ananassa Duch cv. Asia and used to synthesize cDNA by reverse transcriptase enzyme, which was applied in qPCR with primers designed for S-adenosyl-L-methionine synthases (FaSAMS1-3), S-adenosyl-L-methionine decarboxylases (FaSAMDC1-3), arginine decarboxylase (FaADC), spermidine synthase (FaSPDS), 1-aminocyclopropane-1-carboxylate synthases (FaACS1-4) and 1-aminocyclopropane-1-carboxylate oxidase (FaACO1-4) gene sequences. Based on the comparison made between the expression patterns of ethylene- and polyamine-related genes and those of the common precursor- and pathway shift-related genes, it appeared that with the exception of FaSAMS2, FaSAMDC1 and FaSAMDC2, the genes involved in polyamine biosynthesis showed decreasing level of expression as ripening proceeded. |
| Keywords: | pACC oxidase, ACC synthase, S-adenosyl-L-methionine, SAM decarboxylase, spermidine synthase |
| *Correspondence: | Erzsébet Kiss, Szent István University, Institute of Genetics, Microbiology and Biotechnology, no 1 Páter Károly St., 2100, Gödöllő, Hungary, e-mail: kiss.erzsebet@mkk.szie.hu |
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