Phenolic content, antioxidant activity and effect on collagen synthesis of a traditional wound healing polyherbal formula

November 17, 2014

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Article Title:	Phenolic content, antioxidant activity and effect on collagen synthesis of a traditional wound healing polyherbal formula
Authors:	Alexandru V., Gaspar A., Savin S., Toma A., Tatia R., Gille E.
Affiliation:	1National Institute of R&D for Biological Sciences, 296, Splaiul Independentei, sector 6, 060031, Bucharest, Romania 2National Institute of R&D for Biological Sciences, „Stejarul” Biological Research Centre, 6, Alexandru cel Bun Street, 610004, Piatra Neamt, Romania
Abstract:	The aim of this study was to investigate the ethanol extracts of four medicinal plants, Achillea millefolium L., Hyssopus officinalis L., Equisetum arvense L. and Echinacea purpurea L. and their polyherbal formula, used in traditional medicine for wound healing. The study analyzed their total phenolics content using Folin-Ciocalteu method and identified the main constituents by HPLC. Their antioxidant activity was evaluated by DPPH and ABTS assays and the formula’s capacity to enhance collagen synthesis in L929 fibroblast cell culture was determined by Sircol assay. The results showed that the polyherbal extract had phenolic constituents with pharmacological properties: chlorogenic acid, caffeic acid, luteolin and apigenin. It was showed that the polyherbal formula presented higher antioxidant activity than plant extracts and induced a stimulation of collagen synthesis by fibroblasts, which could contribute to wound strength. In conclusion, the proposed polyherbal formula demonstrated high potential as therapeutic agent in wound healing.
Keywords:	polyherbal formula, wound healing, antioxidant activity, collagen, medicinal plants
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*Correspondence:	Valentina Alexandru, National Institute of R&D for Biological Sciences, 296, Splaiul Independentei, sector 6, 060031, Bucharest, Romania, Tel/fax: +40-21-2200882, E-mail: valentinaalexandru@yahoo.com Article published: February 2015

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