The anti-leech effect of ethanolic extract of Achillea millefolium l. compared to levamisole and niclosamide on Limnatis nilotica


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Article Title: The anti-leech effect of ethanolic extract of Achillea millefolium l. compared to levamisole and niclosamide on Limnatis nilotica
Authors: Mahmoud B., Fatemeh A., Ahmad A., Hassan H., Bahareh E., Majid G-A, Mahmoud R-K.
Affiliation: 1Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences Khorramabad, Iran
2Islamic Azad University, Science and Research, North Tehran Branch, Tehran, Iran
3Deputy for Food and Drug, Urmia University of Medical Sciences, Urmia, Iran
4Poultry Diseases Department,Veterinary Medicine Faculty, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran
5Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
6Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
Abstract: This experimental trial was designed to evaluate the anti-Limnatis nilotica effect of Achillea millefolium L. ethanolic extract as well as levimisole and niclosamide. In an experimental study the extract of Achillea millefolium L. aerial parts was prepared and then the severity effect of the treatments was recorded and compared with placebo group on L. nilotica as anti-leech assay. The average time of paralysis and death of L. nilotica for Levamisole, niclosamide and Achillea millefolium L. plant were 12.66±5.19 min, 19.22±3.42 min and 90±17 min, respectively. Distilled water was determined as the inert for control group. In this study, it was determined that Achillea millefolium L. plant with an intensity of 3+ have a good anti-leech effect and can be shown to be effective in cases of leech bitings.
Keywords: Leech, Limnatis nilotica, Achillea millefolium L., Ethanolic extract
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*Correspondence: Prof. Mahmoud Rafieian-Kopaei; Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran; Email: rafieian@yahoo.com