Selection of an efficient chondroitin sulfate-liposome delivery system as chondroprotective agent for local inflammation treatment

November 17, 2014

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Article Title:	Selection of an efficient chondroitin sulfate-liposome delivery system as chondroprotective agent for local inflammation treatment
Authors:	Craciunescu O., Moldovan L., Moisei M., Utoiu E., Zarnescu O., Roseanu A., Trif M.
Affiliation:	Department of Cellular and Molecular Biology, The National Institute of Research & Development for Biological Sciences, 296, Splaiul Independentei, sector 6, 060031, Bucharest, Romania The Institute of Biochemistry of the Romanian Academy, 296, Splaiul Independentei, sector 6, 060031,Bucharest, Romania The University of Bucharest, Faculty of Biology, 91-95, Splaiul Independentei, sector 5, 76201, Bucharest, Romania
Abstract:	We aimed to select a biocompatible concentration of chondroitin sulfate loaded in liposomes (L-CS) in a culture of human chondrocytes for its possible use in intra-articular treatment of inflammatory and degenerative disorders. L-CS effect on cell viability and morphology and its anti-inflammatory effect was investigated in cell culture experimental models. Our data demonstrated that 250-500 µg/ml CS from L-CS formulation was the optimal range concentration for in vitro experiments. L-CS was more efficient than free CS in preventing cell morphology changes and inhibiting gelatinases secretion in the culture medium of inflamed cells. In conclusion, CS loaded in liposomes could be efficient an chondroprotective agent for intraarticular treatment of osteoarthritis.
Keywords:	chondroitin sulfate, liposomes, chondrocytes, cytotoxicity, metalloproteinases
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*Correspondence:	Dr. Lucia Moldovan, The National Institute of Research & Development for Biological Sciences, 296, Splaiul Independentei, sector 6, 060031, Bucharest, Romania, Tel/fax: +40-21-2200882, Email: moldovanlc@yahoo.com

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