Comparative study of the antioxidant activity and of the polyphenolic content for Thymus vulgaris leaves and Pinus sylvestris shoots

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Article Title:	Comparative study of the antioxidant activity and of the polyphenolic content for Thymus vulgaris leaves and Pinus sylvestris shoots
Authors:	Marian Butu, Diana Golea, Steliana Rodino, Alina Butu
Affiliation:	National Institute of Research and Development for Biological Sciences, 060031, Splaiul Independentei 296, Bucharest, Romania, University of Agronomic Sciences and Veterinary Medicine, 011464, Mărăşti Blvd. 59, Bucharest, Romania
Abstract:	The aim of this study was to evaluate the antioxidant activity of hydroalcoholic extracts obtained from Thymus vulgaris leaves and shoots of Pinus sylvestris. It was also spectrophotometrically determined polyphenol carboxylic acid concentration expressed in caffeic acid and flavonoids expressed in quercetin concentration. Antioxidant activity was determined spectrophotometrically using the DPPH system to generate free radicals. This was expressed as quercetin milimolar reported to appropriate units of measurement for each sample. The results obtained showed that for the extract obtained from the leaves of Thymus vulgaris the concentration of polyphenolic compounds was higher than that of the Pinus sylvestris shoots extract. In addition, maceration extracts compared with ultrasonic extracts was more effective, the first ones containing a higher amount of antioxidants targeted. Also, after studying the antioxidant activity of the two extracts it was determined a correlation between this and concentration of polyphenolic compounds.
Keywords:	antioxidants, polyphenolic compounds, free radicals, Thymus vulgaris, Pinus sylvestris
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*Correspondence:	Steliana Rodino, National Institute of Research and Development for Biological Sciences, 060031, Splaiul Independentei 296, Bucharest, Romania, Tel/Fax:+04021 220 0880