Preliminary study of antibacterial effect of extracts obtained by different extraction methods

Preliminary study of antibacterial effect of extracts obtained by different extraction methods

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Title: Preliminary study of antibacterial effect of extracts obtained by different extraction methods
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Article_Title: Preliminary study of antibacterial effect of extracts obtained by different extraction methods
Authors: Marian Butu, Carmen Manole, Steliana Rodino, Alina Butu
Affiliation: National Institute of Research and Development for Biological Sciences, 060031, Splaiul Independentei 296, Bucharest, Romania,
University of Agronomic Sciences and Veterinary Medicine, 011464, Mărăşti Blvd. 59, Bucharest, Romania
Abstract: This paper aims to assess the antibacterial effect of some plant extracts in the form of essential oils obtained from lemon peel (Citrus limon), orange peel (Citrus aurantium), cinnamon (Cinnamomum zeylanicum), clove (Syzygium aromaticum), red currant (Ribes rubrum), Elite variety and black currant (Ribes nigrum) Tinker variety. Disc diffusion method was used, thus determining the antibacterial activity of essential oils against Bacillus subtilis isolate ATCC 6633. The results showed that all essential oils used in the experiment have antibacterial potential. The best results were obtained for the essential oil of cinnamon, the inhibition zone diameter being of 31.55 mm. These preliminary data support a future evaluation of potential applications of active compounds in these essential oils for the purpose of using them as natural therapeutic agents.
Keywords: plant extracts, antibacterian activity, extraction methods
References: Ashok Kumar K., Narayani M, Subanthini A, Jayakumar M, Antimicrobial Activity and Phytochemical Analysis of Citrus Fruit Peels -Utilization of Fruit Waste, International Journal of Engineering Science and Technology, 5414 – 5421, 2011
Bourgou S, Rahali FZ, Ourghemmi I, Saidani Tounsi M, Changes of Peel Essential Oil Composition of Four Tunisian Citrus during Fruit Maturation, The Scientific World Journal, Article ID 528593, 10 pages, doi:10.1100/2012/528593, 2012;
Butnariu M, Coradini CZ, Evaluation of Biologically Active Compounds fromCalendula officinalis Flowers using Spectrophotometry, Chemistry Central Journal 6:35 doi: 10.1186/1752-153X-6-35, 2012
Dhanavade MJ, Jalkute CB., Ghosh JC, Sonawane KD, Study Antimicrobial Activity of Lemon (Citrus lemon L.) Peel Extract, British Journal of Pharmacology and Toxicology, Vol. 2(3), 119 -122, 2011
Gupta C, Garg AP, Uniyal RC, Kumari A, Comparative analysis of the antimicrobial activity of cinnamon oil and cinnamon extract on somefood-borne microbes, African Journal of Microbiology Research, Vol. 2(9), 247-251, 2008
Hegazy AE, Ibrahium MI, Antioxidant Activities of Orange Peel Extracts, World Applied Sciences Journal, Vol. 18 (5), 684-688, 2012
Ikuta K, Hashimoto K, Kaneko H, Mori S, Ohashi K, Suzutani T, Anti-viral and anti-bacterial activities of an extract of blackcurrants (Ribes nigrum L.), Journal of Microbiology and Immunology, Vol. 56, 805 – 809, 2012
Inouye S, Takizawa T, Yamaguchi H, Antibacterial activity of essential oils and their major constituents against respiratory tract pathogens by gaseous contact, Journal of Antimicrobial Chemotherapy, vol47 (5), 565 – 573, 2001
Javed S, Javaid A, Mahmood Z, Javaid A, Nasim F, Biocidal activity of citrus peel essential oils against some food spoilage bacteria, Journal of Medicinal Plants Research, Vol. 5(16), 3697 – 3701, 2012
Kamaliroosta L, Gharachorloo M, Kamaliroosta Z, Alimohammad Zadeh K. H., Extraction of cinnamon essential oil and identification of its chemical compounds, Journal of Medicinal Plants Research Vol. 6(4), 609-614, 2012
Liobikas J, Liegiūtė S, Majienė D, Trumbeckaitė S, Bendokas V, Kopustinskienė DM, Šikšnianas T, Anisimovienė N, Antimicrobial activity of anthocyanin-rich extracts from berries of different Ribes nigrum varieties, Journal Horticulture and vegetable growing, Vol. 27 No. 4 59-66, 2008
Matan N, Antimicrobial activity of edible film incorporated with essential oils to preserve dried fish (Decapterus maruadsi), International Food Research Journal, Vol. 19(4), 1733 – 1738, 2012
Nanasombat S, Wimuttigosol P, Antimicrobial and antioxidant activity of spice essential oils, Food Science and Biotechnology, Vol. 20, 45 – 53, 2011
Oprea Eliza, Radulescu V., Balotescu C., et al., 2008, Chemical and biological studies of Ribes nigrum L. buds essential oil, Biofactors Vol. 34, pp. 3 – 12;
Pinto E, Vale-Silva L, Cavaleiro C, Salgueiro L, Antifungal activity of the clove essential oil from Syzygium aromaticum on Candida, Aspergillus and dermatophyte species, Journal of Medical Microbiology, Vol. 58, 1454–1462, 2009,
Prabuseenivasan S, Jayakumar M, Ignacimuthu S, In vitro antibacterial activity of some plant essential oils, BMC Complementary and Alternative Medicine, Vol.6:39, 2006
Roy SD, Bania R, Chakraborty J, Goswami R, Laila R, Ahmed SA, Pharmacognostic, phytochemical, physicochemical property and antimicrobial activity studies of lemon peel oil, J. Nat. Prod. Plant Resour., Vol. 2 (3), 431- 435, 2012
Saraf A, Mishra MS, Sharma K, Antibacterial activity of commercial and wild cinnamon species, Journal of Phytology, Vol. 3(2), 102 – 106, 2011,
Shareef AA, Evaluation of antibacterial activity of essential oils of Cinnamomum sp. and Boswellia sp., Journal of Basrah Researches (Sciences), Vol. 37(5A), 60-71, 2011,
Read_full_article: pdf/23-2013/23-2013/SU23-2-14Butu1.pdf
Correspondence: Steliana Rodino, National Institute of Research and Development for Biological Sciences, 060031, Splaiul Independentei 296, Bucharest, Romania, Tel/Fax:+04021 220 0880

Read full article
Article Title: Preliminary study of antibacterial effect of extracts obtained by different extraction methods
Authors: Marian Butu, Carmen Manole, Steliana Rodino, Alina Butu
Affiliation: National Institute of Research and Development for Biological Sciences, 060031, Splaiul Independentei 296, Bucharest, Romania,
University of Agronomic Sciences and Veterinary Medicine, 011464, Mărăşti Blvd. 59, Bucharest, Romania
Abstract: This paper aims to assess the antibacterial effect of some plant extracts in the form of essential oils obtained from lemon peel (Citrus limon), orange peel (Citrus aurantium), cinnamon (Cinnamomum zeylanicum), clove (Syzygium aromaticum), red currant (Ribes rubrum), Elite variety and black currant (Ribes nigrum) Tinker variety. Disc diffusion method was used, thus determining the antibacterial activity of essential oils against Bacillus subtilis isolate ATCC 6633. The results showed that all essential oils used in the experiment have antibacterial potential. The best results were obtained for the essential oil of cinnamon, the inhibition zone diameter being of 31.55 mm. These preliminary data support a future evaluation of potential applications of active compounds in these essential oils for the purpose of using them as natural therapeutic agents.
Keywords: plant extracts, antibacterian activity, extraction methods
References: Ashok Kumar K., Narayani M, Subanthini A, Jayakumar M, Antimicrobial Activity and Phytochemical Analysis of Citrus Fruit Peels -Utilization of Fruit Waste, International Journal of Engineering Science and Technology, 5414 – 5421, 2011
Bourgou S, Rahali FZ, Ourghemmi I, Saidani Tounsi M, Changes of Peel Essential Oil Composition of Four Tunisian Citrus during Fruit Maturation, The Scientific World Journal, Article ID 528593, 10 pages, doi:10.1100/2012/528593, 2012;
Butnariu M, Coradini CZ, Evaluation of Biologically Active Compounds fromCalendula officinalis Flowers using Spectrophotometry, Chemistry Central Journal 6:35 doi: 10.1186/1752-153X-6-35, 2012
Dhanavade MJ, Jalkute CB., Ghosh JC, Sonawane KD, Study Antimicrobial Activity of Lemon (Citrus lemon L.) Peel Extract, British Journal of Pharmacology and Toxicology, Vol. 2(3), 119 -122, 2011
Gupta C, Garg AP, Uniyal RC, Kumari A, Comparative analysis of the antimicrobial activity of cinnamon oil and cinnamon extract on somefood-borne microbes, African Journal of Microbiology Research, Vol. 2(9), 247-251, 2008
Hegazy AE, Ibrahium MI, Antioxidant Activities of Orange Peel Extracts, World Applied Sciences Journal, Vol. 18 (5), 684-688, 2012
Ikuta K, Hashimoto K, Kaneko H, Mori S, Ohashi K, Suzutani T, Anti-viral and anti-bacterial activities of an extract of blackcurrants (Ribes nigrum L.), Journal of Microbiology and Immunology, Vol. 56, 805 – 809, 2012
Inouye S, Takizawa T, Yamaguchi H, Antibacterial activity of essential oils and their major constituents against respiratory tract pathogens by gaseous contact, Journal of Antimicrobial Chemotherapy, vol47 (5), 565 – 573, 2001
Javed S, Javaid A, Mahmood Z, Javaid A, Nasim F, Biocidal activity of citrus peel essential oils against some food spoilage bacteria, Journal of Medicinal Plants Research, Vol. 5(16), 3697 – 3701, 2012
Kamaliroosta L, Gharachorloo M, Kamaliroosta Z, Alimohammad Zadeh K. H., Extraction of cinnamon essential oil and identification of its chemical compounds, Journal of Medicinal Plants Research Vol. 6(4), 609-614, 2012
Liobikas J, Liegiūtė S, Majienė D, Trumbeckaitė S, Bendokas V, Kopustinskienė DM, Šikšnianas T, Anisimovienė N, Antimicrobial activity of anthocyanin-rich extracts from berries of different Ribes nigrum varieties, Journal Horticulture and vegetable growing, Vol. 27 No. 4 59-66, 2008
Matan N, Antimicrobial activity of edible film incorporated with essential oils to preserve dried fish (Decapterus maruadsi), International Food Research Journal, Vol. 19(4), 1733 – 1738, 2012
Nanasombat S, Wimuttigosol P, Antimicrobial and antioxidant activity of spice essential oils, Food Science and Biotechnology, Vol. 20, 45 – 53, 2011
Oprea Eliza, Radulescu V., Balotescu C., et al., 2008, Chemical and biological studies of Ribes nigrum L. buds essential oil, Biofactors Vol. 34, pp. 3 – 12;
Pinto E, Vale-Silva L, Cavaleiro C, Salgueiro L, Antifungal activity of the clove essential oil from Syzygium aromaticum on Candida, Aspergillus and dermatophyte species, Journal of Medical Microbiology, Vol. 58, 1454–1462, 2009,
Prabuseenivasan S, Jayakumar M, Ignacimuthu S, In vitro antibacterial activity of some plant essential oils, BMC Complementary and Alternative Medicine, Vol.6:39, 2006
Roy SD, Bania R, Chakraborty J, Goswami R, Laila R, Ahmed SA, Pharmacognostic, phytochemical, physicochemical property and antimicrobial activity studies of lemon peel oil, J. Nat. Prod. Plant Resour., Vol. 2 (3), 431- 435, 2012
Saraf A, Mishra MS, Sharma K, Antibacterial activity of commercial and wild cinnamon species, Journal of Phytology, Vol. 3(2), 102 – 106, 2011,
Shareef AA, Evaluation of antibacterial activity of essential oils of Cinnamomum sp. and Boswellia sp., Journal of Basrah Researches (Sciences), Vol. 37(5A), 60-71, 2011,
*Correspondence: Steliana Rodino, National Institute of Research and Development for Biological Sciences, 060031, Splaiul Independentei 296, Bucharest, Romania, Tel/Fax:+04021 220 0880