Immunohistochemical expression of p53 in squamous cell carcinoma with different localization in the area of the head and neck

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Article Title:	Immunohistochemical expression of p53 in squamous cell carcinoma with different localization in the area of the head and neck
Authors:	Elisabeta Maria Vasca, Virgil Vasca, Raluca Amalia Ceausu, Ovidiu Mederle, Pusa Gaje, Tatiana Duminica, Marius Raica, Caius Doros
Affiliation:	Faculty of Medicine, Pharmacy and Dental Medicine, “Vasile Goldis” Western University Arad, Romania “Victor Babes” University of Medicine and Pharmacy, Department of Histology, Angiogenesis Research Center Timisoara, Romania “Victor Babes” University of Medicine and Pharmacy, Department of Maxillofacial Surgery, Timisoara, Romania “Victor Babes” University of Medicine and Pharmacy, ENT Department, Timisoara, Romania
Abstract:	The p53 protein is a transcription factor, which regulates the cell cycle arrest, DNA repair and apoptosis. Head and neck squamous cell carcinoma remain a challenging disease despite intensive clinical research. The aim of this study was to evaluate the expression of p53 in squamous cell carcinomas with different locations in head and neck region. We included in our study 15 patients diagnosed with squamous cell carcinoma from the pharyngeal area (2 cases), larynx (5 cases), the nasal pyramid (1 case), oral cavity (7 cases). Immunohistochemical tehnique used p53, (clone DO-7, RTU, Dako Cytomation, Denmark) like primary antibody, NovoLink Max Polymer Detection System as visualization system, DAB as chromogen, Lille’s haematoxyline for counterstain. p53 immunoreactivity in tumor cells was estimated as percent of positive cells according to this score: 0 (0% positive cells); 1 (<10%); 2 (10-30%); 3 (>30%). Were found the following values of score to the population included in the study: 3 in the 11% cases, 2 in 62% cases, 1 in 18% cases, 0 in 9% cases. The lowest score was found in the case from submandibular area. It was found a heterogeneity of the score in the area of the oral cavity, for the same degree of differentiation: maximum score for the cases coming from the area of the lip and of the hard palate and minimum score for the squamous cell carcinoma of the tongue. These observations can be useful for a new classification of HNSCC which can improve the diagnosis, better stratification and a more targeted therapeutic schemes of these disease.
Keywords:	p 53, squamous cell carcinoma, head, neck
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