Use of unconventional methods to obtain somaclonal variations, with the purpose of creating new potato varieties that are resistant to diseases and pests

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Article Title:	Use of unconventional methods to obtain somaclonal variations, with the purpose of creating new potato varieties that are resistant to diseases and pests
Authors:	Andreea Tican, Nicoleta Chiru, Diana Ivanovici
Affiliation:	Department of vegetal tissue culture National Institute of Research and Development for Potato and Sugar Beet Brasov, Romania
Abstract:	The most common factors which affect somaclonal variations are genotype explant source, in vitro period and cultivation conditions in which the cultureis established. In this research, calli were induced using leaf disk and leafstalks explants of potato cultivars resistant at potato blight and viroses. Calli were induced with different growing regulators (with different concentrations). Having as objective obtaining of lines with resistance at diseases and pest are necessary: callus induction, callus growing, callus multiplication by subculture, controlling organogenesis processes. Plantlets regeneration from callus is influenced by genotype ad medium variant.
Keywords:	somaclonal variations, callus, tissue culture, induction, medium, genotype, type of explant
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*Correspondence:	Andreea Tican, Institutul Naţional de Cercetare Dezvoltare pentru Cartof şi Sfeclă de Zahăr Braşov, str. Fundăturii nr. 2, tel: 0268/476608, fax:0268/476795, e-mail: tican_andreea@yahoo.com