Extraction and characterization of enzymatic activity of dromedary lipases from Souf region (Algeria)
March 6, 2025
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| Article Title: | Extraction and characterization of enzymatic activity of dromedary lipases from Souf region (Algeria) |
| Authors: | Laiche A.T., Benine C. |
| Affiliation: | Laboratory of Biodiversity and application of biotechnology in the agricultural field, Faculty of the Sciences of Nature and Life, University of El Oued, El-Oued 39000, Algeria Department of Biology. Faculty of Sciences of Nature and Life, University of El-Oued, El-Oued 39000, Algeria. Laboratory of Biology, Environment and Health, Faculty of the Sciences of Nature and Life, University of El Oued, El-Oued 39000, Algeria |
| Abstract: | This study aims to extract lipase from four organs of camels in the “Targuie” population; namely: pancreas, abomasum, rumen, and bump fat. The presence of this enzyme was confirmed in the mentioned organs by infrared spectroscopy, and the measurement was carried out on the lipase activity of various extracts using a spectrophotometer. The physicochemical properties of the abomasum, pancreas, and rumen revealed interesting values of proteins, total fat, and humidity, respectively as follows: pancreas. In addition, lipid biochemical analyses showed elevated cholesterol levels of 125.48 mg/dl, triglycerides of 30.45 mg/dl, high-density lipoproteins (HDL) of 48.94 mg/dl, lipoproteins low density (LDL) of 110.2 mg /dl, while the fat content was estimated at 75%. Analysis of the results of the infrared spectra confirmed the presence of lipase with values similar to those of the reference spectrum of the lipase. In addition, the determination of the lipase activity gave the following values: the pancreas 0.1359 IU/g, the stomach 0.1306 IU/g, the fat 0.2306 IU/g, the rumen 0.1508 IU/g, these results show that fat contains the highest amount of lipase activity. Through this study, the mysterious animal: the dromedary; was explored differently for the first time by looking for enzymes (lipase) with interesting catalytic activity. |
| Keywords: | Algeria, camel, enzymatic activity, infrared spectroscopy, lipase. |
| *Correspondence: | Ammar Touhami Laiche, Laboratory of Biodiversity and application of biotechnology in the agricultural field, Department of Biology, Faculty of the Sciences of Nature and Life, University of El Oued, El-Oued 39000, Algeria, Tel: ++656595940, Fax: ++32120714, email: laiche-ammar-touhami@univ-eloued.dz |
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