The importance of ”in vitro” plant genetic resources conservation – potato (Solanum tuberosum L.)
December 26, 2024
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| Article Title: | The importance of ”in vitro” plant genetic resources conservation – potato (Solanum tuberosum L.) |
| Authors: | Cioloca M., Tican A., Popa M., Șerban V., Baciu A., Iliev P. |
| Affiliation: | National Institute of Research and Development for Potato and Sugar Beet, 2 Fundaturii Street, 500470, Brasov, Romania Research and Development Station for Potato, Targu Secuiesc, Romania Scientific – Practical Institute of Horticulture and Food Technologies, Chisinau, Republic of Moldova |
| Abstract: | The traditional method for the conservation of vegetatively propagated species is to maintain field collections that can include a large number of genotypes, representing a wide range of genetic diversity. ”In vitro” conservation of plant genetic resources, with the help of modern biotechnologies, can be a potential option for the improvement of agricultural crops. Slow Growth Storage (SGS) technique is a valid approach to conserve ”in vitro” various vegetatively propagated species by controlling plantlets growth and development, saving storage space, labor and reducing costs. In potatoes, to eradicate viruses and obtain a healthy initial material, we have at our disposal the technique of sampling and ”in vitro” cultivation of meristems. In this study, 6 potato genotypes were tested using the DAS ELISA technique to establish the health status (presence/absence of potato viruses). In four of these genotypes, healthy clones |
| Keywords: | potato, tissue culture, in vitro germplasm conservation, virus eradication, meristems. |
| *Correspondence: | Cioloca Mihaela, National Institute of Research and Development for Potato and Sugar Beet, Brasov, Plant tissue culture laboratory, 2 Fundaturii Street, 500470, Brasov, Romania, Tel. +40-(268)-476795, Fax +40(268)-476608, email:mihaela.cioloca@potato.ro. |
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