The effect of metals on the survival of saprophytic microflora originated from Upper-Tisza and Szamos Rivers in laboratory conditions

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Article Title:	The effect of metals on the survival of saprophytic microflora originated from Upper-Tisza and Szamos Rivers in laboratory conditions
Authors:	Sándor Balázsy, Márta D. Tóth, Nadiya Boyko, Judit L. Halász, László Simon
Affiliation:	1Institute of Biology, College of Nyíregyháza, Nyíregyháza, Hungary, 2Uzhgorod National University, Uzhgorod, Ukraine 3Dept. of Environm., College of Nyíregyháza, Nyíregyháza, Hungary,
Abstract:	The aim of our paper is to obtain more and better information on the ecological and microbiological status of the inundation area of the Upper-Tisza River in both the Hungarian and the Ukrainian region. With these proper data we shall be able to reach the objectives of our work: conservation of the natural resources and values of the cross border region in order to provide and utilize possibilities for common environmental problems with cross border effects in order to protect surface and sub-surface waters, improve water quality, and effective prevention and environmental management of pollutions related problems both Tisza and Szamos Rivers. Therefore we were studied from 2004 to 2007 years in the body of Tisza and Szamos Rivers the changing of saprophytic microflora on the influence of heavy metals in laboratory experiments. We determined the pH, temperatures, heavy metal contents and total number the saprophytic micro-organisms (sampling sites: Rahó, Milota, Vásárosnamény, Dombrád, Csenger) different places in water. We used cultures media (Nutrient, Malata, Yeast, and Mc Conkey agars), biochemical and API (Bio-Meriux) tests, and heavy metals (Cd, Cu, Zn, Pb). The experiments were in 24 and 48 hours and 50, 100, 500 and 1000 mg/kg metals we used. In Tisza and Szamos Rivers the main micro-organisms species to survive the 1000 mg/kg metals (Cd, Cu, Zn, Pb) were: Aeromonas salmonella salmonicida, Agrobacter radiobacter, Candida humicola, Candida inconspicua, Candida lambica, Cryptococcus laurentii, Cryreomonas luteola, Flavobacterium indologenes, Flavobacterium meningosepticum, Neisseria ssp., Pasteurella ssp., Pseudomonas chlororaphis, Pseudomonas vesicularis, Rhodotorula minuta, Trichosporon cutaneum, Trichosporon pullulans, Vibrio metschnikovii, Sphingomonas paucimobilis. Along the Tisza River the saprophytic microflora was changing and the Szamos saprophytic microflora effects the Tisza water body.
Keywords:	water, metals, bacteria, yeast, pollution
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