Reaction comparison of Begonia semperflorens cv. ‘Ambassador’ white phytoinocula minipropagule type in the presence of thidiazuron and/or indolebutyric acid

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Article Title:	Reaction comparison of Begonia semperflorens cv. ‘Ambassador’ white phytoinocula minipropagule type in the presence of thidiazuron and/or indolebutyric acid
Authors:	Julieta – Emilia GERGELY1*, Dorina CACHIŢĂ-COSMA2
Affiliation:	1 University of Oradea, Faculty of Science, Department of Biology. Oradea. Romania 2 “Vasile Goldis” Western University of Arad, Romania
Abstract:	The objectives of this experiment aimed at the reaction testing of Begonia semperflorens cv. ‘Ambassador’ White minicuttings, detached from a primary culture, which was at 30 days from the plant apex of zygote seedling origin obtained by in vitro seed germination. Subcultivation of minipropagule of about 4-5 mm – consisting of three short petiolated leaves– was made on a solidified basic culture medium, Murashige-Skoog (1962), to which, besides vitamins and 30 g / l sucrose, growth regulators were also added (either 1 mg / l thidiazuron (TDZ) or 1 mg / l indolebutyric acid (IBA), or a mixture – equal parts – of the two growth substances). Compared with the evolution of minipropagule inoculated and cultivated on the basic medium without growth regulators, those grown on an medium containing 1 mg / l TDZ showed a very strong caulogenesis and phylogenic capacity. The counterbalance of this cytokinine with a similar amount of IBA was reduced to about half the leaves and buds generation, but stimulated calusogenesis. TDZ’s total elimination from the culture medium strongly decreased the morphogenesis of B. semperflorens cv. ‘Ambassador’ White minipropagule in the subculture.
Keywords:	Begonia semperflorens, thidiazuron, indolebutyric acid, subcultivation, micropropagation
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*Correspondence:	Julieta – Emilia Gergely, University of Oradea, Faculty of Science, Department of Biology, 1 Universităţii Str., zip code: 410087, Oradea, Romania, phone: 0040259408448, e-mail: jromocea@uoradea.ro