Effect of vitamin E on carbonic anhydrase and superoxide dismutase activity – Experimental research in vitro and in vivo

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Article Title:	Effect of vitamin E on carbonic anhydrase and superoxide dismutase activity – Experimental research in vitro and in vivo
Authors:	Camelia Eliza MRAZ1*, Mariana MUREŞAN1, Angela ANTONESCU1, Otilia MICLE1, Annamaria PALLAG2, Marcela COLTĂU3, Ioan PUŞCAŞ3
Affiliation:	1 Department of Preclinical Sciences, Medicine and Pharmacy Faculty, University of Oradea 2 Department of Pharmacy, Medicine and Pharmacy Faculty, University of Oradea 3 City Hospital “Prof.Dr.Ioan Puşcaş” Şimleul Silvaniei, Sălaj
Abstract:	The purpose of this study was to determine the effect of vitamin E on erythrocyte carbonic anhydrase (CA) and superoxide dismutase (SOD) activity in vitro and in vivo. In vitro effect was followed by purified isoenzymes CA I and CA II using vitamin solutions with concentrations between 10-8 and 10-4 M and in vivo in mice erythrocytes from Male Sprague-Dawley breed. Groups of rats were given vitamin E (75 mg/kg/day, i.g.), 3,4-Benzpyren (200 ppm/day, i.g.), Vitamin E + 3,4-Benzpyren (75 mg/kg/day + 200 ppm/day, i.g), and a Control group only Placebo. Measurement of enzymes activity was done by following the hydration reaction of CO2 (stopped-flow method) for CA, respectively the enzymatic inhibition of oxidation of epinephrine to adrenochrome for SOD. In vitro there is a direct activation of CA by dose-response relationship maximum at concentration 10-4 M. Both in vitro and in vivo, vitamin E produced a increase in CA I and CA II activity, and the effect is stronger on CA II isoenzyme. In vivo studies show that vitamin E decreases CA and SOD inhibition caused by Benzpyren.
Keywords:	vitamin, carbonic anhydrase, superoxide dismutase, activity
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*Correspondence:	Mraz Camelia, University of Oradea, Medicine and Pharmacy Faculty, Department of Preclinical Sciences, 1 December St. no. 10, 410068, Oradea, Romania, Tel. +40-(259)- 415 680/157, email: camelia.mraz@yahoo.com