Experimental studies for the evaluation of ischemic preconditioning mechanisms in rat heart

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Article Title:	Experimental studies for the evaluation of ischemic preconditioning mechanisms in rat heart
Authors:	Cristian Romeo Revnic, Carmen Ginghina, Floarea Revnic, Simona Botea
Affiliation:	1 University of Medicine and Pharmacy ”Carol Davila” Bucharest, Romania 2 C.C. Iliescu Institute, Department of Cardiology Bucharest, Romania 3 „Ana Aslan” National Institute of Geriatrics and Gerontology, Department of Cell and Molecular Biology, Bucharest, Romania 4 „Victor Babes” Institute, Department of Molecular Pathology, Bucharest, Romania
Abstract:	Ischemic preconditioning (IPC) induced by administration of brief episodes of ischemia-reperfusion represents a protective mechanism of the heart against prolonged episodes of ischemia. The mechanism of ischemic preconditioning is far from being elucidated; there are hypotheses involving the activation of the family of protein kinase C (PKC) and also the opening of the KATP channels. Our study was designed to investigate the involvement of KATP channels and PCK activation in the mechanism of ischemic preconditioning of rat heart perfused in Langendorff system using activators of PKC (1,2-dioctanoil-sn-glycerol -DOG) and KATP channels (cromakalim) and inhibitors of PKC (cheleritrine-CHE) and of KATP channels (glibenclamide-GLY). There was an improvement of LVDP in ischemic-preconditioned hearts also a reduction of infarct size vs. controls; using the activators and inhibitors of KATP channels and PCK, it has been demonstrated that these two are involved in the pathway of the ischemia-reperfusion mechanism.
Keywords:	ischemic preconditioning, ischemia-reperfusion, infarct size, Protein kinase C, Katp channels
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*Correspondence:	Cristian Romeo Revnic, UMF Carol Davila, Bucharest, Cardiology Department, C.C. Iliescu Institute Bucharest, No. 3 Aleea Dealul Macinului Str, Bl A44, Sc.C, Ap.39 Sector 6, Zip code 77416, Email: kityrom@yahoo.com