The effect of ischemic preconditioning upon molecular and ultrastructural aspects of rat myocardium

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Article Title:	The effect of ischemic preconditioning upon molecular and ultrastructural aspects of rat myocardium
Authors:	Cristian Romeo Revnic1*, Carmen Ginghina2, Maria Caloianu3, Simona Botea4, Floarea Revnic5
Affiliation:	1 University of Medicine and Pharmacy ”Carol Davila” Bucharest, Romania 2 C.C. Iliescu Institute, Department of Cardiology Bucharest, Romania 3 National Institute for Research and Development in Biological Sciences, Department of Electron Microscopy, Bucharest, Romania 4 “Victor Babes” Institute, Department of Molecular Pathology, Bucharest, Romania 5 National Institute of Geriatrics and Gerontology, Department of Cell and Molecular Biology, Bucharest, Romania
Abstract:	The aim of our study was to characterise the Langendorff model of rat heart investigating different durations of ischemia and reperfusion and ischemic precondition protocol (IPC) on myocardial ischemia reperfusion injury to test the potential clinical relevance of ischemic preconditioning (IP) phenomenon on infarct size, to assess genomic DNA integrity and histological and ultrastructural changes in heart muscle. Our results have shown that as ischemia and reperfusion time is extended the level of infarction is also increased. We concluded that 20 minutes stabilisation and 45 minutes ischemia followed by 30 minutes reperfusion seems to provide a compliant platform to study ischemia reperfusion injury. Protection using (ICP) was induced by 2 cycles 5` ischemia and 5` reperfusion prior lo lethal ischemia reperfusion protocols. The reduction in infarct size in IP hearts pointed out that inhibition of apoptosis is one of the mechanisms which participate in electing the increased resistance to ischemia. The potential clinical relevance of IP on ischemia-reperfusion injury is the object of clinical research to find new methods of cardioprotection in humans.
Keywords:	ischemic preconditioning, apoptosis, necrosis, ischemia, reperfusion
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*Correspondence:	Cristian Romeo Revnic,University of Medicine and Pharmacy ”Carol Davila” Bucharest, Romania, email: frevnic@hotmail.com