Evaluation of metabolic status of cancer cells by histoenzymatic and immunohistochemical analysis

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Article Title:	Evaluation of metabolic status of cancer cells by histoenzymatic and immunohistochemical analysis
Authors:	Annamaria Pallag1, Aurel Ardelean2
Affiliation:	1 Faculty of Medicine and Pharmacy, University of Oradea, Romania 2 ”Vasile Goldiş” Western University Arad, Romania
Abstract:	In the past few years the medical treatment of malignant diseases has steadily increased in scope and importance. Monitoring the effects of this treatment is very important both of study of the influence on the cell metabolism. Mitochondria are subcellular organelles, whose function is to produce ATP through the oxidative phosphorylation system. Alterations in respiratory activity appear to be a general feature of malignant cells. The aim of the present study is to analysis and evaluation of metabolic status of cancer cells by use histoenzymatic techniques to determine the activity of the ATPase, LDH, and immunohistochemical analysis of cytochrome c release, both in tissue samples were obtained before the antineoplastic treatment and after antineoplastic treatment.
Keywords:	cytochrome c, cancer cells, ATPase, LDH
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*Correspondence:	Annamaria Pallag, Faculty of Medicine and Pharmacy, University of Oradea,Oradea, P-ţa 1 Decembrie, nr. 10, jud. Bihor; e-mail: annamariapallag@gmail.com