Immunohistochemistry method preceded by tissue transfer – A reliable alternative to current practice

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Article Title:	Immunohistochemistry method preceded by tissue transfer – A reliable alternative to current practice
Authors:	Denisa Anderco1*, Elena Lazar2, Angela Borda3, Sorina Taban2, Felix Mic1, Andrada Loghin3, Alis Dema2
Affiliation:	1Department of Pathology, Emergency County Hospital, Timisoara, Romania 2Department of Pathology, University of Medicine and Pharmacy Victor Babes, Timisoara, Romania 3Department of Histology, University of Medicine and Pharmacy Targu Mures, Romania
Abstract:	In the context of increased incidence of the prostate cancer, the pathologists are more frequently faced with the task to establish a diagnosis on quantitatively reduced biopsy specimens. Sometimes the usual diagnostic methods do not allow a definite morphological diagnosis due to the limited nature of suspicious lesions and inability to evaluate its architecture. In addition, the loss of these atypical foci from the paraffin blocks during repeated sections is not an isolated phenomenon. In this regard, we reassessed the cases with equivocal diagnoses established on prostate biopsy specimens in our hospital over the past 2 years. We performed the tissue transfer on charged slides, followed by AMACR/p63 cocktail immunostaining in order to establish a certain diagnosis. Following this procedure we established a final diagnosis in 17 of the 19 cases (89.47%). We claim that this method is a reliable alternative to the classical processing of minute atypical glandular lesions, when minimum two H&E–stained sections that harbour suspicious foci are available.
Keywords:	prostate biopsy; diagnosis; immunohistochemistry; AMACR/p63 cocktail
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*Correspondence:	Anderco Denisa, Department of Pathology, Timisoara Emergency County Hospital, Iosif Bulbuca Street no. 10, Timisoara 300736, Timis, Romania, email: denisa_dobre@yahoo.com