Citotoxic effects of three species of Epilobium (Onagraceae) herbal extracts in rats

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Article Title:	Citotoxic effects of three species of Epilobium (Onagraceae) herbal extracts in rats
Authors:	Ioana ROMAN*, Mircea A. RUSU, Constantin PUICĂ, Maria BORŞA
Affiliation:	Animal Physiology Laboratory, Institute of Biological Research, Cluj-Napoca, Romania
Abstract:	Experiments were performed on albino male Wistar rats that were treated with three Herbal extracts of Epilobium hirsutum, Epilobium angustifolium and Epilobium parviflorum (Onagraceae), with a rich content in flavonoids. We investigated the possible cytotoxic effects of the hydro-alcoholic extracts on some metabolic – endocrine – immunologic organs (brain, hypophysis, adrenals, liver, kidney, thymus and spleen) with biochemical, histological and histoenzymological methods. There were no cytotoxic effect. Epilobium hirsutum, which has the best content in flavonoids, has had the most favorable effects.
Keywords:	herbal extracts, vital organs, epilobium, rats
References:	Weiss R., Fintelmann C. Herbal medicine. Thieme Verlag, Stuttgard-New York, 2000 Stănescu U, Miron A, Hăncianu M, Aprotosoaie C., Plante medicinale de la A la Z. Vol. I, Ed. Gr. T. Popa, Iaşi, 2004. Tămaş M, Oniga I, Benedec D, Florian S., Ghid pentru recunoaşterea şi recoltarea plantelor medicinale. Edit. Dacia, Cluj-Napoca., 2005. Rusu MA, Tămaş M, Roman Ioana, Produse fitoterapeutice testate în toxicoze hepatice experimentale. Ed. Todesco. Cluj-Napoca, 2007. Brown L, Cohen M., Calendula. In: Herbs and Natural Supplements. Edit. Elsevier Australia, 170-179, 2007. Hierman A., Jua H, Reidlinge M, Samet W., Isolation of the antiphlogistic principle from Epilobium angustifolium. Planta Med., 57 (4), 357-360, 1991. Arredondo MF, Blasina F, Echeverry C, Morquio A, Ferreira M, Abin-Carriquiry JA, Lafon L, Dajas F., Cytoprotection by Achyrocline satureioides (Lam.) D.C. and some of its main flavonoides against oxidative stress. J. Ethnopharmacol., 91 (1), 13-20, 2004. Ducrey B, Marston A, Göhring S, Hartmann RW, Hostettmann K., Inhibition of 5-alpha-reductase and romatase by the ellagitonnins oenothein A and oenothein B from Epilobium species. Planta Med., 63 (2), 111-114, 1997. Okuda T., Yoshida T., Hatano T., Ellagitannins as active constituents of medicinal plants. Planta Med., 55 (2), 117-122. 1989. Vitalone A, Bordi F, Baldazzi C, Mazzanti G, Saso L, Tita B., Antiproliferative effect on a prostatic epitelial cell line (PZ-HPV-7) by Epilobium angustifolium. Farmaco., 56 (5-7), 483-489, 2001. Vitalone A, mccoll J, Thome D, Costa LG., Tita B., Characterization of the effect of Epilobium extracts on human cell proliferation. Pharmacology, 69 (2), 79-87, 2003. Ivancheva G, Manolova N, Serkedjieva J, et al., Polyphenols from Bulgarian medicinal plants with antiinfectious activity. Basic Life Sci., 59, 717-728, 1992. Voynova E, Voynova E, Dimitrova S, Naydenova E, Karadjov P., Inhibitory action of extracts of Maclura aurantiaca and Epilobium hirsutum on tumor models in mice. Acta Physiol. Pharmacol. Bulg., 17, 50-52, 1991. Willoherb MJ., Epilobium angustifolium: biology, chemistry, biochemistry, bioactivity and uses. Agro Food Industry Hi-Tech, 18-22, 2002. Reitmann S, Frankel S., A colorimetric method for the detrmination of serum glutamic oxaloacetic and glutamic pyruvic transaminases. Amer. J. Clin. Pathol., 28, 56-63, 1957. Wilbur K M, Bernheim F, Shapiro O W., The thiobarbituric acid reagent as a test for oxidation of fatty acids by various agents. Arch. Biochem. Biophys., 24: 305–313, 1949. Zak B, Dickenman RC, White EG, Burnett H, Cherney PJ., Rapid estimation of free and total cholesterol. Am. J. Clin. Pathol., 24, 1307-1315, 1954. Mureşan E, Gaboreanu M., Bogdan AT, Baba AI., Tehnici de histologice normală şi patologică. Ed. Ceres, Bucureşti, 1974. Snedecor G., Cochram W., Statistical methods (6th edn.). Iowa State University Press, Ames, Iowa, 1978. Weber R., Grundiss der biologischen für Naturisben-safteter. Landwirte und Mediziner. G-Fischer Werloag, Jena, 1980. Steenkamp V, Gouws MC, Gulumian M, Elgorashi EE, van Staden J., Studies on antibacterial anti-inflamatory and antioxidant activity of herbal remedies used in the treatment of benign prostatic hyperplasia and prostatis. J. Ethnopharmacol., 103 (1), 71-75, 2006. Rusu MA, Crăciun C, Rusu ML Puică C., Xenobiotice şi radicali liberi cu tropism hepatic. Hepatectomia chimică. Ed. Eikon, Cluj-Napoca, 2005.
*Correspondence:	Ioana Roman, Institute of Biological Research, 48 Republicii Str., PC 400015, Cluj-Napoca, Romania E-mail: danutzroman@yahoo.com