In vitro screening of potato varieties for drought stress induced with different osmotic agents
January 21, 2022
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| Article Title: | In vitro screening of potato varieties for drought stress induced with different osmotic agents |
| Authors: | Tican A., Cioloca M., Bărăscu N., Bădărău C., Hermeziu M. |
| Affiliation: | 1National Institute of Research and Development for Potato and Sugar Beet Brasov,
2 Fundaturii str., 500470 Brasov, Brasov County, Romania 2Transilvania University, Faculty of Food and Tourism Brasov, 148 Castelului str., 500014 Brasov, Brasov County, Romania |
| Abstract: | The objective of this study was to identify potato varieties with tolerance to the drought stress, by introducing in culture medium of different osmotic regulators (mannitol, sorbitol and polyethylene glycol) with various concentrations (0%, 1% and 1.5%). The experiment was performed for three potato Romanian varieties (Marvis, Castrum and Ervant). The determinations were made for different growth and development parameters: plantlets length, number of leaves/plantlets, root length, fresh plantlet and root weight. The nutrient medium with mannitol had as effect reducing of plantlets height (6.93 cm), root length (4.41 cm) and fresh plantlets weight (0.104 g), and PEG (polyethylene glycol) had a negative influence over leaf number (8.33) and weight of the fresh root (0.040 g). The concentration of 1.5% osmotic agent drastically reduced the number of leaves (7.63), the height of plantlets (5.52 cm) and fresh weight of plantlets and root (0.087 g and 0.033 g). |
| Keywords: | potato, in vitro, hydric stress, osmotic regulators, drought |
| *Correspondence: | Tican Andreea, National Institute of Research and Development for Potato and Sugar Beet Brasov, 2 Fundaturii str., 500470 Brasov, Brasov County, Romania; e-mail: tican_andreea@yahoo.com |
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