In silico alpha-amylase enzyme inhibition and in vivo attenuation of intestinal glucose uptake by the N-acetyl cysteine (NAC) in experimental diabetes
January 21, 2022
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| Article Title: | In silico alpha-amylase enzyme inhibition and in vivo attenuation of intestinal glucose uptake by the N-acetyl cysteine (NAC) in experimental diabetes |
| Authors: | Sekiou O., Kherfane W., Honcharova O., Boumendjel M., Bouras-Bouslama A., Frissou N., Mliki F., Benselhoub A., Bouslama Z., Messarah M. |
| Affiliation: | 1 Environmental Research Center (C.R.E), Campus, Sidi Amar, Annaba 23001; Algeria 2 Laboratory of Geodynamics and Natural Resources, Department of Hydraulics, Badji Mokhtar Annaba University, P.O. Box 12, 23000 Annaba. Algeria 3 Kherson State Agrarian and Economic University, Ukraine 4 Laboratory of Biochemistry and Environmental Toxicology (L.B.T.E), Faculty of Sciences, University of Badji Mokhtar, BP 12 Sidi Amar, Annaba, Algeria 5 University of Badji Mokhtar (UBMA), Sidi Amar, Annaba, Algeria 6Laboratory of Fundamental Computer Science Operational Research Combinatory and Econometrics (L’IFORCE) Faculty of Mathematics, University of Sciences and Technology Houari Boumedienne, Algiers, 16111.Algiers. Algeria 7 Laboratory of Terrestrial and aquatic systems ecology (ECOSTAQ) University of Badji Mokhtar, Sidi Amar, Annaba, Algeria |
| Abstract: | In the present study, we investigate the inhibition of the alpha-amylase enzyme and the antioxidant properties of N-acetyl Cysteine (NAC) in alloxan-induced experimental diabetes in rats. After one month of acclimatization in a room with controlled temperature (22±3 °C) and lighting (12-h light/dark cycle), rats divided into four groups of seven animals each: Group I: Control group (C); Group II: Untreated-diabetic group (UD) induced by injection of freshly dissolved alloxan in 1.0ml of sodium citrate buffer (0.1M, pH4.5) in the intraperitoneal way (i.p), at a rate of 150mg/kg body weight; Group III: Positive Control (PC+) treated orally (gavage) by N-acetyl-cysteine,150mg/kg/b.w; Group IV: Treated diabetic group (TD) by N-acetyl-cysteine (150mg /kg/b.w/day) orally along with the experimental protocol. Diabetes led to increased blood glucose beyond the baseline values. During diabetes situations, the total cholesterol, triglycerides, and malondialdehyde were significantly increased. On the contrary, the antioxidants enzymes activities (Catalase, Superoxide Dismutase, Glutathione Peroxidase, and Glutathione-S-Transferase) were decreased. The administration of NAC significantly improved the studied parameters. These results demonstrate that NAC ameliorates hyperglycemia, hyperlipidemia, and oxidative damage in the diabetes situations. In conclusion, the results obtained suggest that NAC supplementation reduces free radical generation, potentiates the antioxidant defense system, and attenuates intestinal glucose uptake. |
| Keywords: | Experimental diabetes, N-acetyl Cysteine, Antioxidants enzymes, Alpha-amylase, Free radical |
| *Correspondence: | Dr. Sekiou Omar, Environmental Research Center (C.R.E), Campus, Sidi Amar, Annaba 23001; Algeria. E-mail address: sekiouomar@yahoo.fr ; o.sekiou@cre.dz Tel: +21377686778 |
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