The Diversity Of Papaya (Carica Papaya) Seeds Indigenous Yeasts And Its Antimicrobial Activities Towards E.Coli And S.Typhimurium
February 17, 2020
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| Article Title: | The Diversity Of Papaya (Carica papaya) Seeds Indigenous Yeasts And Its Antimicrobial Activities Towards E.Coli And S.Typhimurium |
| Authors: | Utama G. L., Herlina, Kayaputri I. L., Balia R. L. |
| Affiliation: | Faculty of Agro-Industrial Technology, Universitas Padjadjaran, Sumedang, Indonesia 45363 Centre for Environment and Sustainability Science, Bandung, Indonesia 40134. Faculty of Animal Husbandry, Universitas Padjadjaran, Sumedang, Indonesia 45363 |
| Abstract: | Papaya seeds has various utilization that can be obtained from its bioactive compound and indigenous microorganism activity. The aim of this study is to identify the diversity of yeasts isolated from papaya seeds and determine the antimicrobial activity towards E.coli and S.typhimurium. Indigenous yeast isolated from papaya seeds using Modified Potato Dextrose Agar (PDA) which is added by yeast extract (Kraft foods) and antibiotics (Amoxicillin). The microscopic and macroscopic characterization has been done by observing shape, texture, color, and size. The species of indigenous yeasts are rapidly checked by using RapID Yeast Plus System that confirmed through the Electronic Code Compendium (www.remel.comeric). The testing of antimicrobial activity of indigenous yeast towards pathogenic bacteria were done by using plug method. The research done experimentally and all of the data were analyzed descriptively. The results showed that there are seven yeasts isolated which three species identified from papaya seeds. The isolates are Candida krusei (1 strain), Candida tropicalis (5 strains) and Cryptococcus albidus (1 strain). Antimicrobial test found that all of the isolate shown antimicrobial activities against E. coli and S. tyhphimurium, except Candida krusei. Candida tropicalis1 shown the highest antimicrobial activities against E. coli, while Candida tropicalis5 has the highest antimicrobial activities towards S. typhimurium. |
| Keywords: | Papaya seeds, Identification, Isolation, Antimicrobial |
| *Correspondence: | Gemilang Lara Utama, Faculty of Agro-Industrial Technology, Universitas Padjadjaran, Jl. Raya Bandung Sumedang Km. 21, Jatinangor, West Java, Indonesia 45363, e-mail : g.l.utama@unpad.ac.id |
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