Experimental study of erythrocyte membranes in rats orally exposed to caffeinated energy drinks by fluorescent probe technique
February 17, 2020
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| Article Title: | Experimental study of erythrocyte membranes in rats orally exposed to caffeinated energy drinks by fluorescent probe technique |
| Authors: | Posokhov Y. O., Tkachenko A. S., Nakonechna O. A., Onishchenko A. I., Korniyenko Y. M., Tkachenko M. O. |
| Affiliation: | The National Technical University “Kharkiv Polytechnic Institute”, Kharkiv, Ukraine Kharkiv National Medical University, Kharkiv, Ukraine V.N. Karazin Kharkiv National University, Kharkiv, Ukraine Kharkiv Municipal Clinical Hospital Nº 27, Kharkiv, Ukraine |
| Abstract: | The aim of our research was to assess the state of red blood cell (RBC) membranes in rats orally administered caffeinated energy drinks during two months using the fluorescent probe O1O (2-(2-OH-phenyl)-5-phenyl-1,3-oxazole). Fluorescent probe O1O (2-(2-OH-phenyl)-5-phenyl-1,3-oxazole), which locates in the area of glycerol backbones, carbonyl groups of phospholipids, and hydrocarbon chains of phospholipids (near carbonyl groups) in the bilayer, was used to assess the state of RBC membranes in suspensions prepared from whole blood samples obtained from ten female adult WAG rats orally administered a caffeinated energy drink at a dose of 12 ml/kg during two months and ten controls. Energy drink consumption was associated with a higher fluorescence intensity of the phototautomer form of the probe O1O (the fluorescence maximum is at a wavelength of 480 nm) in erythrocyte suspensions compared with the control animals. The observed change in the fluorescence of the probe is attributed to the increase in the viscosity of the probe environment in the membrane. It was revealed using the fluorescent probe O1O that the long-term oral administration of caffeinated energy drinks to rats caused the increased membrane viscosity (i.e. reduced fluidity) in RBCs. |
| Keywords: | caffeinated energy drinks, fluorescent probe, rats, cell membrane |
| *Correspondence: | Anton Tkachenko, Kharkiv National Medical University,, Department of Biochemistry, Nauky ave 4, 61022, Kharkiv, Ukraine, Tel. +38050-109-45-54, Fax. +38(057)700-41-32, email: antontkachenko555@gmail.com |
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