New articleDevelopment by flow cytometry of bioassays based on chlorella for environmental monitoring
November 17, 2014
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| Article Title: | Development by flow cytometry of bioassays based on chlorella for environmental monitoring |
| Authors: | Petrescu C-M, Turcus V., Mihali C-V, Stana I.O., Bratosin D. |
| Affiliation: | 1 Institute of Life Sciences, ”Vasile Goldiș” Western University of Arad, Romania 2 Faculty of Medicine, Department of Biology, ”Vasile Goldiș” Western University of Arad, Romania 3 National Institute for Biological Science Research and Development, 296 Spl. Independentei, 060031-Bucharest, Romania |
| Abstract: | In ecotoxicological assessments, bioassays (ecotoxicity tests or biotests) are one of the main tools, defined as methods which use living cells, tissues, organism or communities to assess exposure-related effects of chemicals. The increasing complexity of environmental degradation requires an increase in the capacity of scientific approach in monitoring and notification as early as possible risks. Our own objective concerns the detection of aquatic environment pollution in Romania and particularly in the Danube basin. For assessing aquatic environment pollution degree or for assessing cytotoxicity or ecotoxicity of pollutants (heavy metals, nanoparticles, pesticides, etc.) we developed news experimental bioassays based on the use of viability and apoptosis biomarkers of Chlorella cells by flow cytometry. Our proposed bioassays could be rapid and very sensitive tests for in laboratory aquatic risk assessment and biomonitoring. |
| Keywords: | bioassays, aquatic pollution, cytometry, biomonitoring |
| *Correspondence: | Constantin-Marian Petrescu, Institute of Life Sciences, ”Vasile Goldiș” Western University of Arad, Romania, marian.petrescu@yahoo.com |
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