Influence of acidic extracellular pH from preservative medium on intracellular pH during storage in SAGM additive solution
November 17, 2014
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| Article Title: | Influence of acidic extracellular pH from preservative medium on intracellular pH during storage in SAGM additive solution |
| Authors: | Tusa I., Rugina A., Stan L., Toader A.L., Calu L., Enache M., Iordachel C., Bratosin D. |
| Affiliation: | 1National Institute for Biological Science Research and Development, Bucharest, Romania 2Vasile Goldis” Western University of Arad, Faculty of Medicine, Arad, Romania |
| Abstract: | Erythrocytes (RBCs) transfusion is a crucial treatment to save lives in case of illness or severe anemia caused by chemotherapy or blood loss due to trauma, accident or major surgery. All currently licensed additive solutions for RBCs have an acidic pH (approx. 5.6-5.8), which is far below the normal physiological pH of 7.3 of venous blood. Because blood sampling is done in an anticoagulant medium with an acidic pH, we aimed to study by flow cytometry, the morphology and physiology changes induced by acid pH. The results of our researches revealed the negative influence of the extracellular acid pH on the much earlier and faster appearance of storage lesions on red blood cells. We have demonstrated the efficiency and sensitivity of flow cytometry methods used in assessing the quality of stored components. |
| Keywords: | RBCs, intracellular pH, storage conditions, blood banks, flow cytometry, scanning electron microscopy |
| *Correspondence: | Daniela Bratosin, National Institute for Biological Science Research and Development, Bucharest, Romania; Vasile Goldis” Western University of Arad, Faculty of Medicine, Biology Department, Arad, Romania, email: danielabratosin@gmail.com |
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