Comparative evaluation of storage lesions induced by anticoagulant medium on blood for transfusion
November 17, 2014
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| Article Title: | Comparative evaluation of storage lesions induced by anticoagulant medium on blood for transfusion | ||
| Authors: | Tusa I., Toader A.L., Calu L., Rugina A., Stan L., Enache M., Iordachel C., Bratosin D. | ||
| Affiliation: | 1National Institute for Biological Science Research and Development, Bucharest, Romania 2Vasile Goldis” Western University of Arad, Faculty of Medicine, Biology Department, Arad, Romania | ||
| Abstract: | Numerous cellular changes occur during RBCs storage in blood banks. Morphological and physiological changes resulting from the storage of RBCs are known as “storage lesions”. These alterate RBCs normal biology and functionality, affects both oxygen transport efficiency and other additional risks of disruption of the immune system and coagulation. The similarity between red blood cells senescence phenotype and one of cells in apoptosis, rise to the hypothesis that the accelerated RBCs senescence may be an apoptosis phenomenon even for the particular case of human erythrocytes, cells lacking in mitochondria and nucleus. Thus, we evaluated the storage lesions induced by anticoagulant medium of RBCs for transfusion. The results of our researches revealed the involvement of an apoptosis phenomenon in accelerated aging of red blood cells in blood bags as the basic phenomenon of storage lesions. Also, our results have validated our new proposed cell viability and apoptosis detection assays by flow cytometry to explore the quality of stored blood components for transfusion after blood storage. | ||
| Keywords: | RBCs, transfusion, storage lesions, apoptosis, ROS, viability, flow cytometry | ||
| *Correspondence: | Daniela Bratosin, National Institute for Biological Science Research and Development, Bucharest, Romania; Vasile Goldis” Western University of Arad, Faculty of Medicine, Biology Department, Arad, Romania, email: danielabratosin@gmail.com |
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