Botanical characterization, phytochemical analysis and antioxidant activity of indigenous red raspberry (Rubus idaeus L.) leaves
November 17, 2014
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| Article Title: | Botanical characterization, phytochemical analysis and antioxidant activity of indigenous red raspberry (Rubus idaeus L.) leaves |
| Authors: | Costea T., Vlase L., Gostin I.N., Olah N.K.,Predan G.M.I. |
| Affiliation: | 1 Pharmacognosy, Phytochemistry, Phytotherapy Department, Faculty of Pharmacy, “Carol Davila University” of Medicine and Pharmacy, Bucharest, Romania 2 Biopharmaceutical Department, Faculty of Pharmacy, “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania 3Faculty of Biology, “Alexandru Ioan Cuza” University, Iasi, Romania 4SC Plantextrakt SRL, Quality Research and Development Department, Rădaia 407059, Cluj-Napoca, Romania 5 Department of Botany and Microbiology, Faculty of Biology, University of Bucharest, Bucharest, Romania |
| Abstract: | In the present study, the botanical profile, chemical composition and antioxidant activity of indigenous red raspberry (Rubus idaeus L.) leaves were investigated. The microscopic characterization was determined by means of scanning electronic microscopy and transverse sections. Polyphenols, sterolic compounds and ascorbic acid were determined using thin layer chromatography, spectrophotometric and HPLC assays. The scavenger activity against 2,2-diphenyl-1-picrylhydrazyl free radical and the reducing power capacity were used for antioxidant activity evaluation. It was found that raspberry leaves are a source of tannins (9.88 g%), flavonoids (0.50 g%), phenolcarboxylic acids (1.13 g%) and sterols (0.89 g%). The HPLC assays reaveled a considerable amount of quercitrin (15.63 mg%), p-coumaric acid (17.55 mg%), ferulic acid (4.88 mg%) and ascorbic acid (4.39 g%). The antioxidant activity was weak compared to standard reference (chlorogenic acid). The herbal product is a source of natural compounds with moderate antioxidant activity. |
| Keywords: | polyphenols, sterols, tannins, antioxidant capacity, scanning electron microscopy. |
| *Correspondence: | Teodora Costea, “Carol Davila” University of Medicine and Pharmacy, Faculty of Pharmacy, Pharmacognosy, Phytochemistry, Phytoterapy Department, no.6, Traian Vuia Street, Bucharest, Romania, telephone: 0040722554665, email: teodoracostea85@yahoo.com |
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