Biomarkers of oxidative stress, lipid peroxidation and ros production induced by TiO2 microparticles on snails Helix aspersa
November 17, 2014
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| Article Title: | Biomarkers of oxidative stress, lipid peroxidation and ros production induced by TiO2 microparticles on snails Helix aspersa | ||
| Authors: | Khene L., Berrebbah H., Yahyaoui A., Bouarroudj T., Zouainia S., Kahli H., Bourayou C. | ||
| Affiliation: | 1Laboratory of Cellular Toxicology, Department of Biology, Faculty of Science, Badji Mokhtar University, Annaba, Algeria 2Laboratory of Chemistry Inorganic Materials (LCMI). Département of Chemistry. Faculty of Science, Badji Mokhtar University, Annaba, Algeria | ||
| Abstract: | The increasing production of nanomaterials will in turn increase the release of nanosized by products to the environment. The aim of this study was to assess toxicity of TiO2 microparticle on the bioindicator model snail Helix aspersa. Gastropods are exposed to microparticle TiO2 with Semi-static method at several concentrations (31.25; 62.5; 125; 250; 500; 1000 mg/kg of soil) mixed to ISO soil (ISO11268-1) for 28 days. Oxidative stress biomarkers: Antioxidant enzymatic activities (CAT and GST) were investigated, level of Glutathione (GSH), Lipid peroxidation indicator malondialdehyde (MDA), and free radical was quantified. TiO2 microparticle exposure has led to a significantly induction of antioxidant enzymes Catalase (CAT), glutathione-S-transferase (GST) which might be associated with decreased level of Glutathione (GSH) and lipid peroxidation supported by a significant increase in (MDA) level. It can be hypothesized that by the triggering of oxidative stress by TiO2 microparticles, level of free radicals was quantified on snail and the measuring of ROM’s on snails hemolymph confirm the high production of ROS. | ||
| Keywords: | Helix aspersa, TiO2 microparticle, Biomarkers, Oxidative stress , ROS. | ||
| *Correspondence: | Lyes Khene, Laboratory of Cellular Toxicology, Department of Biology, Faculty of Science, Badji Mokhtar University, Annaba, Algeria. e-mail: khene.lyes@yahoo.com , Tel: +213550857818. |
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