Assessment of exposure wheat Triticum aestivum L. to zinc oxide nanoparticles (ZNO): Evaluation of oxidative damage
November 17, 2014
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| Article Title: | Assessment of exposure wheat Triticum aestivum L. to zinc oxide nanoparticles (ZNO): Evaluation of oxidative damage |
| Authors: | Yahyaoui A., Djebar M.R., Khene L., Bouarroudj T., Kahli H., Bourayou C. |
| Affiliation: | 1Laboratory of Cellular Toxicology, Department of Biology, Faculty of Science, Badji Mokhtar University, Annaba, Algeria. 2Laboratory of Chemistry Inorganic Materials (LCMI). Département of Chemistry. Faculty of Science, Badji Mokhtar University, Annaba, Algeria. |
| Abstract: | Nanoparticles (NPs) are introduced in a growing number of commercial products and their production may lead to their release in the environment. To increase knowledge on Zinc oxide nanoparticles accumulation and impact on plants, we designed a study on wheat Triticum aestivum L. Plants were exposed to ZnO-NPs at several concentrations (10; 50; 100; 500; 1000; 1500; 2000 ppm) for 14 days. ZnO nanoparticles were prepared by chemical synthesis method. The crystal structure of the ZnO was investigated by using, x-ray diffraction (XRD). This impact have been studied on roots and leaves by measuring the plant growth, Oxidative stress biomarkers (CAT) and (APX) activities, Oxidative damage indicators such as lipid peroxidation (MDA) and (H2O2) content, as well respiratory energy metabolism was examined. Our results highlight a decrease of growth wheat plants and reveals a significantly induction of antioxidant enzymatic activities such as CAT and APX. We noted also a lipid peroxidation supported by a significant dose-related increase in MDA and H2O2 level and a significant inhibition of respiratory activity. Triggering of the endogenous antioxidant system and the inhibition of mitochondrial respiratory activity could be explained by the increased enhance production of free radicals causing cellular damage. |
| Keywords: | Triticum aestivum L., Zinc nanoparticles, Oxidative stress, Phytotoxicity, Respiratory metabolism. |
| *Correspondence: | Amina Yahyaoui, Laboratory of Cellular Toxicology, Department of Biology, Faculty of Science, Badji Mokhtar University, Annaba, Algeria. Email: amina.yahyaoui@yahoo.com |
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