SEM and EDAX morphological observation of three ornamental species of the Genus Magnolia

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Article Title:	SEM and EDAX morphological observation of three ornamental species of the Genus Magnolia
Authors:	Nicoleta-Valentina Groza, Ciprian-Valentin Mihali, Violeta Turcus, Aurel Ardelean
Affiliation:	Faculty of Natural Sciences, Engineering and Informatics, “Vasile Goldiş” Western University, Arad, Romania Institute of Life Science, “Vasile Goldiş” Western University, Arad, Romania
Abstract:	The purpose of this study is to reveal certain preliminary aspects related to the morphology and ultrastructure of vegetative and reproductive organs in three species of Magnolia. The study will be extended by quantifying the particles of dust or other impurities adhering to the leaves, so as to show the amount of pollution characteristic of the areas from which the plant materials were sampled and are to be investigated. For that purpose, we will proceed by identifying the presence of certain reference metals by means of EDAX analyses. The detailed description of both structure and ultrastructure in Magnolia species is intended as relevant so as to enable a comparison to previous researches regarding the decorative trees/shrubs in question. Its purpose is to facilitate communication among researchers and practitioners, in the event that they wish to undertake studies at tissue, cell or even molecular level. For the purposes of generating a detailed description, several studies were carried out via optical microscopy and scanning electron microscopy. Scanning electron microscopy (SEM) will allow for in-depth study, resulting in an ample examination of the structure of vegetative and reproductive organs of trees yielding special ornamental value. This preliminary study is to be completed by pursuing the analysis of the other vegetative organs. The Magnolia kobus flower has much more particularities than that of the hybrids: a greater number of stamens, carpels, ovary chambers, pollen grains; this species also presents with poorly pigmented reproductive organs. Following the micro-elemental EDAX analysis of organic matter represented by leaves collected from different species of the genus Magnolia – Magnolia kobus, Magnolia x soulangeana, Magnolia liliiflora -, we were able to determine the presence or absence of those chemical elements which present a potential polluting effect. The chemical elements of significance in urban pollution which were determined by EDAX analysis were Al and Cl.
Keywords:	Magnolia kobus, Magnolia x soulangeana, Magnolia x “Susan”, Magnolia liliiflora, scanning electron microscopy (SEM), EDAX
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*Correspondence:	Groza N. V., Faculty of Natural Sciences, Engineering and Informatics, “Vasile Goldiş” Western University, Arad, Romania, biognicole@yahoo.com