Cytological investigations of the in vitro plants of Sedum telephium ssp. maximum L. at the root apex

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Article Title:	Cytological investigations of the in vitro plants of Sedum telephium ssp. maximum L. at the root apex
Authors:	Mirela Ardelean, Dorina Cachiţă-Cosma, Adrian Marcel Buruiană
Affiliation:	“Vasile Goldiş” Western University from Arad, Life Sciences Institute, Vegetal Biotechnology Department,Arad, Romania “Vasile Goldiş” Western University from Arad, Life Sciences Institute, Vegetal Biotechnology Department, Arad, Romania “Vasile Goldiş” Western University from Arad, Faculty of Natural Sciences, Arad, Romania
Abstract:	While analyzing the results regarding the reaction of plant inoculum of Sedum telephium ssp. maximum L. produced by the propagules cultivated in the medium of Murashige – Skoog (1962) (MB – MS) with an input of different growth regulators, which stood at the basis of the experiments carried out within the monitorisation of the reactions of the explants of Sedum telephium ssp. maximum L., at the presence in the cultivation sub-layer that we modified, we have noticed -at the root apexes of the plants cultivated in MB – MS mediums with input of different growth regulators, using 1,5 mg/l from each of them-, a raspberry-redcoloration of the apexes of the small roots regenerated at the level of different types of explants, either propagules, or callus regenerated from the propagules. These particular reactions observed at the level of plant inoculum of Sedum telephium ssp. maximum L. determined us to lead our investigations towards the examination of these cells with an optical microscope. After the examination with an optical microscope of the root apexes of the in vitro plants of Sedum telephium ssp. maximum L. or of the small roots regenerated at the level of callus, we inferred the fact that, both the calyptra cells and the meristem (situated under the apex) have been coloured in red, especially in the case of the plant inoculum cultivated in the MB – MS medium, with a supplement of the mixture KIN and ANA (1,5mg/l from each of them). We highlighted the presence of these anthocyanins only at the level of plant inoculum of Sedum telephium ssp. maximum L., as this interesting phenomenon was not observed in the case of the organs belonging to plants cultivated in natural conditions. The presence of anthocyanins in the root apexes is a novelty in the specialty literature.
Keywords:	Sedum telephium ssp. Maximum L., root, in vitro, plants, cytology
References:	Andrei M., Paraschivoiu R.M, 2003. Microtehnică botanică [Micro-technical Botany]. Bucharest: The Niculescu Publishing House. Cachiţă, C.D., Ardelean, A., 2009, Tratat de biotehnologie vegetală [Handbook of Vegetal Biotechnology], vol. II, Dacia Publishing House, Cluj-Napoca, p. 32 – 116. Chung, K.-T., Tit, Y.W., Cheng, I.W., Yao-Wen, H., Yuan, L., 1998, Tannins and human health: a review. Critical Reviews in Food Science and Nutrition 38 (6), 421 – 464. Hahn, D.H., 1984. Phenols of sorghum and maize: the effect ofgenotype and alkali processing. Ph.D. dissertation. Texas A&M University, College Station, TX. Hahn, D.H., Rooney, L.W., 1986, Effects of genotype on tannins and phenols of sorghum, Cereal Chemistry 63, 4 – 8. Hahn, D.H., Rooney, L.W., Faubion, J.M., 1983. Sorghum phenolic acids, their HPLC separation and their relation to fungal resistance. Cereal Chemistry 60, 255 – 259. Lev-Yadun, A., Gould, K.S., 2009, Role of anthocyanins in plant defence. J.Theor. Biol. 244. p. 279 – 289. Metcalfe C.R. & Chalk L. 1972. Crassulaceae, 1: 578-581 in Anatomy of the Dicotyledons. Oxford: Clarendon Press. Moţa C., Roşu A., Câmpeanu Gh. (2004) – Compuşi bioactivi de origine vegetală. Abordări biotehnologice. [Bioactive Compounds of Vegetal Origin. A Biotechnical Approach] Câmpeanu Gh., Dumitru I.F. (eds)., Progrese în biotehnologie, Publishing House of the University of Bucharest, Vol. II, p. 99-126; Mulinacci A.N., Vincieri F.F. şi Wagner R. 1993. Anti-inflammatory and immunologically active polysaccharides of Sedum telephium. Phytochemistry, 34:1357-1362. Murashige T. şi Skoog F. 1962. A revised medium for rapid growth and bioassays with tabaco tissue culture. Physiol. Plant, 15: 473-497. Oberthur, E.E., Nicholson, R.L., Butler, L.G., 1983. Presence of polyphenolic materials, including condensed tannins, in sorghum callus. J Agric Food Chem. 31(3): 660 – 2. Qu, J., Zhang, W., Yu, X., 2011, Instability of anthocyanin composition under different subculture conditions during long-term suspension cultures of Vitis vinifera L. var. Gamay Fréaux. Sheng Wu Gong Cheng Xue Bao; 27 (11):1613 -22. Rodrigues, R.F., da Silva, P.F., Shimizu, K., Freitas, A.A., Kovalenko, S.A., Ernsting, N.P., Quina, F.H., Maçanita, A., 2009, Ultrafast internal conversion in a model anthocyanin-polyphenol complex: implications for the biological role of anthocyanins in vegetative tissues of plants.Chemistry. 2009;15 (6): p. 397 – 402. Şerbănescu-Jitariu G, Andrei M., Mitroiu-Rădulescu N şi Petria E. 1983. Practicum de biologie vegetală. [Practicum of Vegetal Biology] Bucharest: the Ceres Publishing House. Ştefan N. şi Oprea A. 2007. Botanică sistematică [Systematic Botany]. Iaşi: Publishing House of the Univ. “Alexandru Ioan Cuza”, 552 pp. Waniska, R.D., 2000. Structure, phenolic compounds and antifungal proteins of sorghum cariopses In: Technical and institutional options for sorghum grain mould management: proceedings of an international consultation.
*Correspondence:	Mirela Ardelean, „Vasile Goldiş” Western University of Arad, Life Sciences Institute, Vegetal Biotechnology Department, 86, L. Rebreanu, Arad, Romania, phone: 0040257228622 fax: 0040257228622, e-mail: mirela.ardelean1@yahoo.com