Study about the ultrasound effects on potato plantlets (Solanum tuberosum L.), conserved in vitro through slow growth method

October 9, 2012

Study about the ultrasound effects on potato plantlets (Solanum tuberosum L.), conserved in vitro through slow growth method

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Title:	Study about the ultrasound effects on potato plantlets (Solanum tuberosum L.), conserved in vitro through slow growth method
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Article_Title:	Study about the ultrasound effects on potato plantlets (Solanum tuberosum L.), conserved in vitro through slow growth method
Authors:	Manuela Ibanescu, Dana Constantinovici, Silvia Strajeru
Affiliation:	Banca de Resurse Genetice Vegetale Suceava
Abstract:	In this paper an attempt to investigate the effects of ultrasounds on potato conserved by in vitro technique is presented. All experiments were carried out at 35 KHz frequency of ultrasounds for 14 minutes exposure time and two different slow growth media. A combination of these parameters with a lower temperature in the conservation room allowed an extension of period between two subcultures from 18 to 40 months, on the same medium, without loosing the viability and vitality of the plantlets.
Keywords:	anatomy, stem, leaf, woody climber, Lonicera pallida
References:	Aladjadjiyan A, The use of physical methods for plant growing stimulation in Bulgaria. Journal of Central European Agriculture Vol 8 (2007) No 3, pp. 369-380, 2007 Cachiţă CD, Metode ”in vitro” la plantele de cultură, Ed. Ceres, Bucureşti, 1987 Constantinovici D, In vitro growth responses and plant storage in old local potato populations, in „15 ani de învăţământ horticol în Banat”, Editura Agroprint Timişoara, pp. 53-58, 2005 Espinoza N, Lizarraga R, Siguenas C, Buitron F, Bryan J, Dodds HJ, Tissue culture: Micropropagation, conservation, and export of potato germplasm. International Potato Center (CIP) Research Guide 1, Lima, Peru, 19, 1992 Murashige T., Skoog F., A revised medium for rapid growth and bioassay with tobacco tissue cultures, Physiologia Plantarum 15, pp. 473-497, 1962 Shors JD, Soll DR, Daniels KK, Gibson DP, Method for enhancing germination. University of Iowa Research Foundation, Assignee, US patent 5,950,362. September 14, 1999 Toledo J, Espinoza N, şi Golmirzae, Tissue Culture Management of in Vitro Plantlets in Potato Seed Production, Trening Manual, Lima, Peru, 1998 Weinberger P, Burton C, The effect of sonication on the growth of some tree seeds. Can J Forestry Research, 11, pp. 840-844, 1981 Westcott R.J., Tissue culture storage of potato germplasm. 1. Minimal growth storage. Potato Research. 24, pp. 331-342, 1981 Westcott R.J., Tissue culture storage of potato germplasm. 2. Use of growth retardants. Potato Research. 24, pp. 343-352, 1981
Read_full_article:	pdf/18-2008/SU08Ibanescu.pdf
Correspondence:	Dana CONSTANTINOVICI, Banca de Resurse Genetice Vegetale Suceava, B-dul 1 Mai, nr 17, 720224, Suceava, Romania, tel +0230 52 41 89; e-mail: dana_constantinovici@yahoo.com

	Read full article
Article Title:	Study about the ultrasound effects on potato plantlets (Solanum tuberosum L.), conserved in vitro through slow growth method
Authors:	Manuela Ibanescu, Dana Constantinovici, Silvia Strajeru
Affiliation:	Banca de Resurse Genetice Vegetale Suceava
Abstract:	In this paper an attempt to investigate the effects of ultrasounds on potato conserved by in vitro technique is presented. All experiments were carried out at 35 KHz frequency of ultrasounds for 14 minutes exposure time and two different slow growth media. A combination of these parameters with a lower temperature in the conservation room allowed an extension of period between two subcultures from 18 to 40 months, on the same medium, without loosing the viability and vitality of the plantlets.
Keywords:	anatomy, stem, leaf, woody climber, Lonicera pallida
References:	Aladjadjiyan A, The use of physical methods for plant growing stimulation in Bulgaria. Journal of Central European Agriculture Vol 8 (2007) No 3, pp. 369-380, 2007 Cachiţă CD, Metode ”in vitro” la plantele de cultură, Ed. Ceres, Bucureşti, 1987 Constantinovici D, In vitro growth responses and plant storage in old local potato populations, in „15 ani de învăţământ horticol în Banat”, Editura Agroprint Timişoara, pp. 53-58, 2005 Espinoza N, Lizarraga R, Siguenas C, Buitron F, Bryan J, Dodds HJ, Tissue culture: Micropropagation, conservation, and export of potato germplasm. International Potato Center (CIP) Research Guide 1, Lima, Peru, 19, 1992 Murashige T., Skoog F., A revised medium for rapid growth and bioassay with tobacco tissue cultures, Physiologia Plantarum 15, pp. 473-497, 1962 Shors JD, Soll DR, Daniels KK, Gibson DP, Method for enhancing germination. University of Iowa Research Foundation, Assignee, US patent 5,950,362. September 14, 1999 Toledo J, Espinoza N, şi Golmirzae, Tissue Culture Management of in Vitro Plantlets in Potato Seed Production, Trening Manual, Lima, Peru, 1998 Weinberger P, Burton C, The effect of sonication on the growth of some tree seeds. Can J Forestry Research, 11, pp. 840-844, 1981 Westcott R.J., Tissue culture storage of potato germplasm. 1. Minimal growth storage. Potato Research. 24, pp. 331-342, 1981 Westcott R.J., Tissue culture storage of potato germplasm. 2. Use of growth retardants. Potato Research. 24, pp. 343-352, 1981
*Correspondence:	Dana CONSTANTINOVICI, Banca de Resurse Genetice Vegetale Suceava, B-dul 1 Mai, nr 17, 720224, Suceava, Romania, tel +0230 52 41 89; e-mail: dana_constantinovici@yahoo.com

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