Spleen structural damages and tissular biochemical parameters disturbances at wistar rats after chimiotherapy administration

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Article Title:	Spleen structural damages and tissular biochemical parameters disturbances at wistar rats after chimiotherapy administration
Authors:	Anca Hermenean, Aurel Ardelean, Constantin Crăciun
Affiliation:	1 Faculty of Medicine, “Vasile Goldiș” Western University, Arad, Romania 2 Electron Microscopy Center, “Babes-Bolyai” University, Cluj-Napoca, Arad, Romania
Abstract:	The Cyclophosphamide (CFA) is a frequently used citostatic drug in the anti-cancer chemotherapy. Unfortuntaley, the administration of the drug is accompanied by side effects which mainly affect the lymphoid organs. In this experiment, we have analyzed the Wistar rats spleen structural and ultrastructural changes for 24 hours after the CFA 3 consecutive administration and the ability of its morphology recovering after 10 days of finished treatment. The 24 hours spleen histopathological and electron microscopy examination after CFA last dose administration demonstrated the installations of some major structural changes especially at the white pulp level in a necrobiosis and apoptosis different lymphocytes stages, the loss of intercellular contacts. Spleen morphological alterations, although profound, are transitory. Since 10 days from the CFA therapy ending, the spleen starts to recover, a fact demonstrated mainly by the compensatory hematopiesis launching. In conclusion, the immune system normal morphofunctionality resume represents a drug theraphy cancer reoccuring.
Keywords:	chemotherapy, spleen morpho-functional damages, reversible side effects
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*Correspondence:	Anca Hermenean, Department of Histology, Faculty of Medicine, “Vasile Goldiş” Western University, 91-94 Revolution Avenue, Arad, Romania, e-mail: anca.hermenean@gmail.com