Vitrocultures of Drosera rotundifolia L. preserved in Suceava gene bank

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Article Title:	Vitrocultures of Drosera rotundifolia L. preserved in Suceava gene bank
Authors:	Violeta Turcuş, Dana Constantinovici, Dorina Cachiţă-Cosma, Adela Halmágyi
Affiliation:	1 “Vasile Goldiş” Western University, Arad, Romania 2 Suceava Gene Bank 3 The Institute for Biological Research in Cluj-Napoca
Abstract:	We monitored the evolution of Drosera rotundifolia L. vitroplantules regenerated from propaguls, consisting of leafs rosettes, inoculated in fresh growth media that we produced. The phytoinoculs of D. rotundifolia, maintained in a “slow growth” conditions, either on Murashige – Skoog (1962) basic media, supplemented with manithol 30g/l or using daminozyde (B9) 30mg/l, kept their viability to subcultures, regenerating new plantules, and the colonies directly transferred to a septic media, in casolettes, being planted in an non sterile substrate made of flower soil and black peat managed to survive. Macroscopic investigations carried out on stereomicroscope as well as those on the scanning electron microscope favoured our encompassing of the regeneration phenomenon produced at the level of the superior epidermis of some leaf lamina of the microrosettes of new leafs, especially those situated at the extremities of the propaguls colony.
Keywords:	Drosera rotundifolia, preserved vitroculture, propaguls
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*Correspondence:	Violeta Turcuș, „Vasile Goldis” Western University, Arad, Department of Plant Biology, email: violeta.turcus@gmail.com