In vitro reactivity of Cymbidium hybridum L. protocorms, on bistratified culture mediums, using bidistilled water or glucose solutions as supernatants

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Article Title:	In vitro reactivity of Cymbidium hybridum L. protocorms, on bistratified culture mediums, using bidistilled water or glucose solutions as supernatants
Authors:	Cristian-Felix Blidar, Ildikó Szabó, Adriana Petruş-Vancea, Liviu Pop
Affiliation:	1 University of Oradea, Faculty of Science, Department of Biology, Romania 2 University of Oradea, Faculty of Medicine and Pharmacy, Department of Botany, Romania
Abstract:	Knowing the fact that the protocorms multiplication processes are accelerated in their submersion conditions in liquid medium, against the situation that, these protocorms are vitrocultivated on solid (agarized) medium cultures (which prevails in organogenesis processes), our objective is to study the influence exerted by the cultures, practiced in bistratified regime, to Cymbidium protocorms vitrocultures. In this interest, we used bidistilled water as supernatant, either on 2% or 5% glucose, which were applied over the inoculated protocorms on agarized medium cultures. The basic medium culture used by us in these experiments was Murashige – Skoog (1962). To this, we added different growth regulators, such as: 2,4-D (2 mg/l), or mixture of BA (2 mg/l) with NAA (1 mg/l), or only BA (2 mg/l), or only NAA (1 mg/l). The witness lot was consisted of vitrocultivated protocorms on agarized medium culture, without growth regulators, cultivated in monolayer. After 90 days from the initiation of double-layer medium cultures, we ascertained that, the application of the second layer (the liquid one) over the agarized medium cultures, strongly stimulated the multiplication of Cymbidium protocorms, mostly if the second layer was bidistilled water; the usage of a solution of glucose 2% as supernatant on medium culture without growth regulators, was better in comparison with the control variant, the neoformed protocorms, being numerical above 1.8 times more.
Keywords:	protocorms, Cymbidium (orchid), double-layer, glucose, in vitro
References:	Blidar CF, Cachiţă-Cosma D, Evoluţia protocormilor de Cymbidium pe medii de cultură bistratificate, în regim hormonal variat. In: Manifestare jubiliară “25 de ani de culturi de ţesuturi în România”, Lucr. celui de al X-lea Simp. Naţ. de Cult. de Ţes. şi Cel. Veg., Cachiţă, C.D., Rakosy, T.L., Ardelean, A. (Ed.), Edit. Risoprint, Cluj-Napoca, 195-201, 2002. Blidar CF, Cachiţă-Cosma D, Reacţia protocormilor de Cymbidium hybridum pe medii de cultură bistratificate, utilizând soluţie de zaharoză ca supernatant. In: Lucr. celui de al XI-lea Simp. Naţ. de Cult. de Ţes. şi Cel. Veg., (ed.: Cachiţă-Cosma D, Ardelean A), Edit. Daya, Satu-Mare, 178-184, 2003. Blidar CF, Cachiţă-Cosma D, Petruş-Vancea A, Pop L., Marian M, The Cymbidium hybridum protocorms reaction, cultivated „in vitro” on a bistratified medium, using a fructose solution as supernatant. In: Internat. Conf. „Agricultura land food industries within the context of european integration”, Edit. „Lucian Blaga” University of Sibiu, 37-48, 2007. Cachiţă-Cosma D, Contribuţii la tehnica de multiplicare şi programare „in vitro” a orhideelor. In: Lucr. celui de al II-lea Simp. Naţ. de Cult. de Ţes. şi Cel. Veg. „in vitro”, Acad. de Şt. Agr. şi Silvice, Bucureşti, vol. I, 191-197, 1982. Cachiţă-Cosma D, Metode in vitro la plantele de cultură. Bazele teoretice şi practice. Bucureşti, Edit. Ceres: 274 pp, 1987. Molnár Gy, Hungarian Patent. no. 183978, 1982. Molnár Gy, Symposium „in vitro” Problems Related to Mass Propagation of Horticultural Plants. Gembloux (Belgium), Book of Abstracts, 1, 1985. Murashige T, Skoog F, A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol., 15: 473-479, 1962. Pătru D, Cachiţă-Cosma D, Floriş C., Studii referitoare la evoluţia „in vitro” a protocormilor de Cymbidium hybridum în cazul substituirii zaharozei din mediile de cultură, cu fructoză. Analele Univ. Oradea Fasc. Biologie, Tom IX, 235-240, 2002.
*Correspondence:	Cristian-Felix BLIDAR, University of Oradea, Faculty of Science, Biology Department, no. 1 Universitatii St., 410087 Oradea, Romania, tel.: +40.259.408.278, fax: +40.259.408.461, e-mail: cricrianb2003@yahoo.co.uk, cblidar@uoradea.ro