Enzyme activities in the preluvosoil of Oradea


Enzyme activities in the preluvosoil of Oradea

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Title: Enzyme activities in the preluvosoil of Oradea
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Article_Title: Enzyme activities in the preluvosoil of Oradea
Authors: Alina Dora Samuel, Monica Şipoş
Affiliation: University of Oradea, Department of Plant Biology, Oradea, Romania
Abstract: Soil enzyme activities (actual and potential dehydrogenase, catalase, acid and alkaline phosphatase) were determined in the 0–20–, 20–40– and 40–60–cm layers of a preluvosoil submitted to a complex tillage (no-till and conventional tillage), crop rotation (2– and 6–crop rotations) and fertilisation [mineral (NP) fertilisation and farmyard-manuring] experiment. Each activity in both non-tilled and conventionally tilled soil under all crops of both rotations decreased with increasing sampling depth. No-till – in comparison with conventional tillage – resulted in significantly higher soil enzymatic activities in the 0–20– and in significantly lower activities in the deeper layers. The soil under maize or wheat was more enzymeactive in the 6– than in the 2–crop rotation. In the 2–crop rotation, higher enzymatic activities were recorded under wheat than under maize. In the 6–crop rotation, the enzymatic indicators of soil quality decreased, depending on the nature of crops and kind of fertilisers (mineral NP or farmyard manure), in the following
order: minerally fertilised (m.f.) wheat > m.f. oats-clover > farmyard-manured maize > m.f. soybean > m.f. clover> m.f. maize. This order means that by determination of enzymatic activities valuable information can be obtained regarding fertility status of soils. It should be emphasised that farmyard-manuring of maize – in comparison with its mineral (NP) fertilisation – led to a significant increase in each of the five enzymatic activities determined. There were positive correlations between acid phosphatase activity and mobile phosphorus content under maize and wheat crops of the both rotations.
Keywords: catalase, crop rotation, dehydrogenase, phosphatase, tillage
References: Angers DA, Bissonnette N, Légére A, Samson N, Microbial and bio- chemical changes induced by rotation and tillage in a soil under barley production. Can. J. Sci., 73, 39-50, 1993.
Balota EL, Kanashiro M, Filho AC, Andrade DS, Dick RP, Soil enzyme activities under long-term
tillage and crop rotation systems in subtropical agro-ecosystems. Brazilian J. of Microbiology,
35, 300-306, 2004.
Bandick AK, Dick RP, Field management effects on soil enzyme activities. Sol. Biol. Biochem., 31,
1471-1479, 1999.
Breakwell DP, Turco RF, Nutrient and phytotoxic contributions of residues to soil in no-till
continuous corn ecosystems. Biol Fertil., 8, 328-334, 1990.
Canarutto S, Mazzoncini M, Perna A, Cervelli S, The effect of reduction of inputs on phosphatase
activity, organic carbon content and water stability index in a corn cultivated soil. Fresenius Environ. Bull., 4, 291-296, 1995.
Clarholm M, Rosengren-Brinck U, Phosphorus and nitrogen fertilization of a Norway spruce foresteffects on needle concentrations and acid phosphatase activity in the humus layer. Plant
Soil, 175, 239-249, 1995.
Deng SP, Tabatabai MA, Effect of tillage and residue management on enzyme activities in soils. III
Phosphatases and arylsulfatase. Biol. Fertil. Soils., 24, 141-146, 1997.
Dick RP, A review: long-term effects of agricultural systems on soil biochemical and microbial
parameters. Agric. Ecosyst. Environ., 40, 25-36, 1992.
Dick RP, Soil enzyme activities as integrative indicators of soil health, In: Pankhurst CE, Doube BM, Gupta VVSR, (Eds.). Biological Indicators of Soil Health CAB International, pp. 121-156, 1997
Dick RP, Myrold DD, Kerle EA, Microbial biomass and soil enzyme activities in compacted and
rehabilitated skid trail soils. Soil Sci. Soc. Am. J., 52, pp. 512-516, 1988
Dick RP, Şandor JA, Eash NS, Soil enzyme activities after 1500 years of terrace agriculture in the
Colca Valley, Peru. Agric. Ecosyst. Environ., 50, pp. 123-131, 1994.
Domuţa C, Agrotehnica terenurilor în pantă din nordvestul României. Ed. Univ. Oradea, pp. 66-117,
2005.
Drăgan-Bularda M, Lucrări practice de microbiologie generală. Univ. Babeş-Bolyai, Cluj-Napoca, pp.
163-167, 1983.
Farrell RE, Gupta VVSR, Germida JJ, Effects of cultivation on the activity and kinetics of
arylsulfatase in Saskatchewan soils. Soil. Biol. Biochem., 26, pp. 1033-1040, 1994.
Fenn LB, Tipton JL, Tatum G, Urease activity in two cultivated and non-cultvated arid soils. Biol.
Fert. Soils, 13, pp. 152-154, 1992.
Griffith DR, Kladivko EJ, Mannering JV, West TD, Parsons SD, Long-tillage and rotation effects on
corn growth and yield on high and low organic matter, poorly drained soil. Agron. J., 80, 599-605, 1988.
Gupta VVSR, Germida JJ, Distribution of microbial biomass and its activity in different soil
aggregate size classes as affected by cultivation. Soil. Biol. Biochem., 20, 777-786, 1988.
Kiss S, Paşca D, Drăgan-Bularda M, Cristea V, Blaga G, Crişan,R, Muntean V, Zborovschi E, Mitroescu S, Enzymological analysis of lead and zinc mine spoils submitted to biological recultivation. Stud. Univ. Babeş-Bolyai, Biol., 35 (2), 70-79, 1990.
Őhlinger R, Phosphomonoesterase activity with the substrate phenylphosphate, In: Schinner F, Őhlinger R, Kandeler E, Margesin, R., (Eds.). Methods in Soil Biology. Springer, Berlin, p. 210-213, 1996a.
Őhlinger R, Dehydrogenase activity with the substrate TTC, In: Schinner F, Őhlinger R, Kandeler E,
Margesin R, (Eds.), Methods in Soil Biology. Springer, Berlin, pp. 241-243, 1996b.
Samuel AD, Kiss S, The effects of soil management practices on the enzymatic activities in a brown
luvic soil. Stud. Univ. Babeş-Bolyai, Biol. 44, (1-2), pp. 189-197, 1999.
Samuel AD, Kiss S, Sandor M, Phosphatase activities in a brown luvic soil. Stud. Univ. Babeş-Bolyai,
Biol. 45, (2), pp. 91-99, 2000.
Ştefanic G, Enzimologia solurilor cultivate, In: Kiss S, Ştefanic G, Paşca D, Drăgan-Bularda M,
Zborovschi E, Crişan R, Enzimologia mediului înconjurător, vol. 1. Ceres, Bucureşti, pp. 65-84, 1991.
Read_full_article: pdf/18-2008/SU08Samuel.pdf
Correspondence: Alina Dora Samuel, University of Oradea, Department of Plant Biology, email: samuelalina@rdslink.ro

Read full article
Article Title: Enzyme activities in the preluvosoil of Oradea
Authors: Alina Dora Samuel, Monica Şipoş
Affiliation: University of Oradea, Department of Plant Biology, Oradea, Romania
Abstract: Soil enzyme activities (actual and potential dehydrogenase, catalase, acid and alkaline phosphatase) were determined in the 0–20–, 20–40– and 40–60–cm layers of a preluvosoil submitted to a complex tillage (no-till and conventional tillage), crop rotation (2– and 6–crop rotations) and fertilisation [mineral (NP) fertilisation and farmyard-manuring] experiment. Each activity in both non-tilled and conventionally tilled soil under all crops of both rotations decreased with increasing sampling depth. No-till – in comparison with conventional tillage – resulted in significantly higher soil enzymatic activities in the 0–20– and in significantly lower activities in the deeper layers. The soil under maize or wheat was more enzymeactive in the 6– than in the 2–crop rotation. In the 2–crop rotation, higher enzymatic activities were recorded under wheat than under maize. In the 6–crop rotation, the enzymatic indicators of soil quality decreased, depending on the nature of crops and kind of fertilisers (mineral NP or farmyard manure), in the following
order: minerally fertilised (m.f.) wheat > m.f. oats-clover > farmyard-manured maize > m.f. soybean > m.f. clover> m.f. maize. This order means that by determination of enzymatic activities valuable information can be obtained regarding fertility status of soils. It should be emphasised that farmyard-manuring of maize – in comparison with its mineral (NP) fertilisation – led to a significant increase in each of the five enzymatic activities determined. There were positive correlations between acid phosphatase activity and mobile phosphorus content under maize and wheat crops of the both rotations.
Keywords: catalase, crop rotation, dehydrogenase, phosphatase, tillage
References: Angers DA, Bissonnette N, Légére A, Samson N, Microbial and bio- chemical changes induced by rotation and tillage in a soil under barley production. Can. J. Sci., 73, 39-50, 1993.
Balota EL, Kanashiro M, Filho AC, Andrade DS, Dick RP, Soil enzyme activities under long-term
tillage and crop rotation systems in subtropical agro-ecosystems. Brazilian J. of Microbiology,
35, 300-306, 2004.
Bandick AK, Dick RP, Field management effects on soil enzyme activities. Sol. Biol. Biochem., 31,
1471-1479, 1999.
Breakwell DP, Turco RF, Nutrient and phytotoxic contributions of residues to soil in no-till
continuous corn ecosystems. Biol Fertil., 8, 328-334, 1990.
Canarutto S, Mazzoncini M, Perna A, Cervelli S, The effect of reduction of inputs on phosphatase
activity, organic carbon content and water stability index in a corn cultivated soil. Fresenius Environ. Bull., 4, 291-296, 1995.
Clarholm M, Rosengren-Brinck U, Phosphorus and nitrogen fertilization of a Norway spruce foresteffects on needle concentrations and acid phosphatase activity in the humus layer. Plant
Soil, 175, 239-249, 1995.
Deng SP, Tabatabai MA, Effect of tillage and residue management on enzyme activities in soils. III
Phosphatases and arylsulfatase. Biol. Fertil. Soils., 24, 141-146, 1997.
Dick RP, A review: long-term effects of agricultural systems on soil biochemical and microbial
parameters. Agric. Ecosyst. Environ., 40, 25-36, 1992.
Dick RP, Soil enzyme activities as integrative indicators of soil health, In: Pankhurst CE, Doube BM, Gupta VVSR, (Eds.). Biological Indicators of Soil Health CAB International, pp. 121-156, 1997
Dick RP, Myrold DD, Kerle EA, Microbial biomass and soil enzyme activities in compacted and
rehabilitated skid trail soils. Soil Sci. Soc. Am. J., 52, pp. 512-516, 1988
Dick RP, Şandor JA, Eash NS, Soil enzyme activities after 1500 years of terrace agriculture in the
Colca Valley, Peru. Agric. Ecosyst. Environ., 50, pp. 123-131, 1994.
Domuţa C, Agrotehnica terenurilor în pantă din nordvestul României. Ed. Univ. Oradea, pp. 66-117,
2005.
Drăgan-Bularda M, Lucrări practice de microbiologie generală. Univ. Babeş-Bolyai, Cluj-Napoca, pp.
163-167, 1983.
Farrell RE, Gupta VVSR, Germida JJ, Effects of cultivation on the activity and kinetics of
arylsulfatase in Saskatchewan soils. Soil. Biol. Biochem., 26, pp. 1033-1040, 1994.
Fenn LB, Tipton JL, Tatum G, Urease activity in two cultivated and non-cultvated arid soils. Biol.
Fert. Soils, 13, pp. 152-154, 1992.
Griffith DR, Kladivko EJ, Mannering JV, West TD, Parsons SD, Long-tillage and rotation effects on
corn growth and yield on high and low organic matter, poorly drained soil. Agron. J., 80, 599-605, 1988.
Gupta VVSR, Germida JJ, Distribution of microbial biomass and its activity in different soil
aggregate size classes as affected by cultivation. Soil. Biol. Biochem., 20, 777-786, 1988.
Kiss S, Paşca D, Drăgan-Bularda M, Cristea V, Blaga G, Crişan,R, Muntean V, Zborovschi E, Mitroescu S, Enzymological analysis of lead and zinc mine spoils submitted to biological recultivation. Stud. Univ. Babeş-Bolyai, Biol., 35 (2), 70-79, 1990.
Őhlinger R, Phosphomonoesterase activity with the substrate phenylphosphate, In: Schinner F, Őhlinger R, Kandeler E, Margesin, R., (Eds.). Methods in Soil Biology. Springer, Berlin, p. 210-213, 1996a.
Őhlinger R, Dehydrogenase activity with the substrate TTC, In: Schinner F, Őhlinger R, Kandeler E,
Margesin R, (Eds.), Methods in Soil Biology. Springer, Berlin, pp. 241-243, 1996b.
Samuel AD, Kiss S, The effects of soil management practices on the enzymatic activities in a brown
luvic soil. Stud. Univ. Babeş-Bolyai, Biol. 44, (1-2), pp. 189-197, 1999.
Samuel AD, Kiss S, Sandor M, Phosphatase activities in a brown luvic soil. Stud. Univ. Babeş-Bolyai,
Biol. 45, (2), pp. 91-99, 2000.
Ştefanic G, Enzimologia solurilor cultivate, In: Kiss S, Ştefanic G, Paşca D, Drăgan-Bularda M,
Zborovschi E, Crişan R, Enzimologia mediului înconjurător, vol. 1. Ceres, Bucureşti, pp. 65-84, 1991.
*Correspondence: Alina Dora Samuel, University of Oradea, Department of Plant Biology, email: samuelalina@rdslink.ro