Phylogenetic relationships of Cyprinidae (Teleostei: Cypriniformes) inferred from the cox1 gene sequences

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Article Title:	Phylogenetic relationships of Cyprinidae (Teleostei: Cypriniformes) inferred from the cox1 gene sequences
Authors:	Catalina Luca, Angelica Andreea Dudu, Adrian Luca, Anca Dinischiotu, Marieta Costache
Affiliation:	1 Biology Molecular Center, University of Bucharest, Romania 2 Faculty of Electronics, Telecommunications and Information Technology, University Politehnica of Bucharest, Romania
Abstract:	The family Cyprinidae is the largest freshwater fish group in the world, including over 200 genera and 2100 species. Mitochondrial DNA investigations have been largely used to explore phylogenetic cyprinid relationships. A fragment of (302bp) from the mitochondrial DNA (mtDNA) subunit I citochrome oxidase gene (cox1) of cyprinid species was sequenced and compared in order to study their phylogenetic relationships. The topologies of neighbor-joining, maximum parsimony and maximum likelihood trees based on cox1 sequences allowed us to identify two major lineages in cyprinids: Cyprininae and Leuciscinae. The Cyprininae group is represented by Carassius auratus auratus and forms a separate branch in the trees. The Leuciscinae group includs Rutilus rutilus, Tinca tinca, Hypophthalmichthys molitrix, Arischthys nobilis, Ctenopharyngodon idella, Leuciscus borysthenicus celensis and Leuciscus cephalus. Misgurnus fossilis was used as outgroup species.
Keywords:	molecular phylogeny, cox1 gene, cyprinid
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*Correspondence:	Costache M., University of Bucharest, Molecular Biology Center, no. 91-95, Spl. Independentei, Bucharest 5, Romania, Tel +40-(21)-3181575; +40-722-683961, email: marietacostache@yahoo.com