Cellular methods for in vitro engineering of human autologous cartilage. A review
October 1, 2012
Cellular methods for in vitro engineering of human autologous cartilage. A review
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Title: | Cellular methods for in vitro engineering of human autologous cartilage. A review |
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Article_Title: | Cellular methods for in vitro engineering of human autologous cartilage. A review |
Authors: | Daniela Bratosin, Catalin Iordachel, Alexandrina Rugina, Ana Maria Gheorghe, Andreea C.I. Ciotec , Nicolae Efimov, Manuela Sidoroff |
Affiliation: | 1 National Institute for Biological Science Research and Development, Bucharest, Romania 2 ”Vasile Goldis” Western University of Arad, Faculty of Biology, Arad, Romania 3 CFR 2 Hospital, Bucharest, Romania |
Abstract: | Articular cartilage repair remains a challenge for regenerative medicine. This review summarizes our research findings that have been undertaken, with a particular emphasis on those techniques that have been introduced into our laboratory, via in vitro and preclinical studies. |
Keywords: | osteoarthritis, chondrocytes, flow-cytometry, tissue engineering, regenerative medicine |
References: | Aigner T., Kim H.A., Apoptosis and cellular vitality: Issues in osteoarthritic cartilage degeneration, Arthritis Rheum., 46:1986-1996, 2002 Aigner T., Kim H.A., H. I. Roach, Apoptosis in osteoarthritis, Rheum. Dis. Clin. N. Am., 30:639-654, 2004 Almarza A.J., Athanasiou K.A., Effects of initial cell seeding density for the tissue engineering of the temporomandibular joint disc. Ann Biomed Eng., 33:943–950, 2005 Aydelotte M.B., Thonar E.J., Mollenhauer J., Flechtenmacher J., Culture of chondrocytes in alginate gel: variations in conditions of gelation influence the structure of the alginate gel, and the arrangement and morphology of proliferating chondrocytes. In Vitro Cell Dev Biol Anim., 34:123–130, 1998 Bratosin D., Estaquier J., Petit F., Arnoult D., Quatannens B., Tissier J.P., Slomianny C., Sartiaux C., Alonso C., Huart J.J., Ameisen J.C., Montreuil J., Programmed cell death in mature erythrocytes: a model for investigating death effector pathways operating in the absence of mitochondria, Cell Death Differ., 8:1143-1156, 2001 Bratosin D., Buia L., Heron A., Montreuil J., US Patent Application 20100256234, Method of stimulating proliferation of differentiated cells belonging to chondrocytes line”, Published on October 7, 2010 Buckwalter J.A., Lane N.E., Athletics and osteoarthritis. Am. J. Sports Med., 25, 873-881, 1997 Bursch W., Ellinger A., Gerner C., Frohwein U., R. Schulte-Hermann, Programmed cell death (PCD). Apoptosis, autophagic PCD or others ?, Ann. N.Y. Acad. Sci., 926:1-12, 2001 Buia-Takacs L., Iordachel C., Efimov N., Caloianu M., Montreuil J., Bratosin D., Flow cytometric analysis of encapsulated chondrocytes cultured in alginate gel for cartilage tissue engineering, Jurnal Medical Aradean (Arad Medical Journal), Vol. XIII, issue 4:25-33, 2010 DeLise A.M., Archer C.W., Benjamin M., Ralphs J.R., Organisation of the chondrocyte cytoskeleton and its response to changing mechanical conditions in organ culture, J. Anat., 194, 343-35, 1999 Fukuda T., Wang H., Nakanishi H., Kosaka K., Yamamoto T., Novel non-apoptotic morphological changes in neurons of the mouse hippocampus following transient hypoxic-ischemia, Neurosci. Res., 33:49-55, 1999 Gold G.E., Bergman A.G., Pauly J.M., Lang P., Butts R.K., Beaulieu C.F., Hargreaves B., Frank L., Boutin R.D., Macovski A., Resnick D., Magnetic resonance imaging of knee cartilage repair. Top Mag Reson Imag., 9, 377-392, 1998 Grandolfo M., D’andrea P., Paoletti S., Martina M., Silvestrini G., Bonucci E., Vittur F., Culture and differentiation of chondrocytes entrapped in alginate gels. Calcif Tissue Int., 52:42–48, 1993 Guo J.F., Jourdian G.W., Maccallum D.K., Culture and growth characteristics of chondrocytes encapsulated in alginate beads, Connect Tissue Res., 19:277–297, 1989 Hamilton D.W., Riehle M.O., Monaghan W., Curtis A.S., Articular chondrocyte passage number: Influence on adhesion, migration, cytoskeletal organisation and phenotype in response to nano- and micro-metric topography. Cell Biol Int., 29: 408–421, 2005 Kerr J.F.R., Wyllie A.H., Currie A.R., Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics, Br. J. Cancer, 26:239- 257,1972 Koch R.J., Gorti G.K., Tissue engineering with chondrocytes. Facial Plast Surg., 18:59–68, 2002 Leist M., Jaattela M., Four death and a funeral: From caspases to alternative mechanisms, Nat. Rev. Mol. Cell Biol., 2:589-598, 2001 Li Y., Ma T., Kniss D.A., Laski L.C., Yang S.T., Effects of filtration seeding on cell density, spatial distribution, and proliferation in nonwoven fibrous matrices. Biotechnol Prog., 17: 935–944, 2001 Liu X., Ma P.X., Polymeric scaffolds for bone tissue engineering. Ann Biomed Eng., 32: 477–486, 2004 Martin J.A., Buckwalter J.A., Aging, articular cartilage chondrocyte senescence and osteoarthritis, Biogerontolog,. 3, 257-264, 2002 Mcfetridge P.S., Bodamyali T., Horrocks M., Chaudhuri J.B., Endothelial and smooth muscle cell seeding onto processed ex vivo arterial scaffolds using 3D vascular bioreactors. ASAIO J., 50:591–600, 2004 Muir H., The chondrocyte, architect of cartilage. Biomechanics, structure, function and molecular biology of cartilage matrix macromolecules, Bioessays, 17, 1039-1048, 1995 Paige K.T., Cima L.G., Yaremchuk M.J., Vacanti J.P., Vacanti C.A., Injectable cartilage. Plast Reconstr Surg., 96:1390–1398, 1995 Price J.S., Waters J.G., Darrah C., Pennington C., Edwards D.R., Donell S.T., Clark I.M., The role of chondrocyte senescence in osteoarthritis, 1, 57-65, 2002 Radisic M., Euloth M., Yang L., Langer L.E., Freed G. Vunjak-Novakovic, High-density seeding of myocyte cells for cardiac tissue engineering. Biotechnol Bioeng., 82: 403–414, 2003 Randolph M.A., Anseth K., Yaremchuk M.J., Tissue engineering of cartilage. Clin Plast Surg., 30:519– 537, 2003 Roach H.I., Clarke N.M.P., Cell paralysis as an intermediate stage in the programmed cell death of epiphyseal chondrocytes, J.Bone Miner Res, 14:1367-1378, 1999 Roach H.I., Clarke N.M.P., Physiological cell death of chondrocytes in vivo is not confined to apoptosis: New observations on the mammalian growth plate, J. Bone Joint Surg., 82-B:601- 613, 2001 Roach H.I., Aigner T., Kouri J.B., Chondroptosis: A variant of apoptotic cell death in chondrocytes ?, Apoptosis, 9:265-27, 2004 Saxon L., Finch C., Bass S., Sports participation, sports injuries and osteoarthritis: Implications for prevention. Sports Med., 28, 123-135, 1999 Shimizu K., Ito A., Honda H., Enhanced cell-seeding into 3D porous scaffolds by use of magnetite nanoparticles. J Biomed Mater Res B Appl Biomater, 77: 265–272, 2006 Soletti L., Nieponice A., Guan J., Stankus J.J., Wagner W.R., Vorp D.A., A seeding device for tissue engineered tubular structures. Biomaterials, 27: 4863–4870, 2006 Sperandio, De Belle I., Bredesen D.E., An alternative, nonapoptotic form of programmed cell death., Proc. Natl. Acad. Sci. USA, 97:14376-14381, 2000 Takacs-Buia L., Iordachel C., Efimov N., Caloianu M., Montreuil J., Bratosin D., Pathogenesis of osteoarthritis: Chondrocyte replicative senescence or apoptosis?, Cytometry Part B (Clinical Cytometry), 74B:356–362,2008 Ushida T., Furukawa K., Toita K., Tateishi T., Threedimensional seeding of chondrocytes encapsulated in collagen gel into PLLA scaffolds. Cell Transplant, 11: 489–494, 2002 Vignon E., Arlot M., Patricot I. M., Vignon G., The cell density of human femoral head cartilage, Clin. Orthop., 121:303-308, 1976 Vujak-Novakovic G., Radisic M., Cell seeding of polymer scaffolds. Methods Mol Biol., 238:131–146, 2004 Yang K.G., Saris D.B., Geuze R.E., Van Rijen M.H., Van Der Helm Y.J., Verbout A.J., Creemers L.B., Dhert W.J., Altered in vitro chondrogenic properties of chondrocytes harvested from unaffected cartilage in osteoarthritic joints. Osteoarthritis Cartilage, 14: 561–570, 2006 Yudoh K., Nguyen T., Nakamura H., Hongo-Masuko K., Kato T., Nishioka K., Potential involvement of oxidative stress in cartilage senescence and development of osteoarthritis: oxidative stress induces chondrocyte telomere instability and downregulation of chondrocyte function, Arthritis Res. Ther., 7, 380-391, 2005 Zhao F., Ma T., Perfusion bioreactor system for human mesenchymal stem cell tissue engineering: Dynamic cell seeding and construct development. Biotechnol Bioeng., 91:482–493, 2005 |
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Article Title: | Cellular methods for in vitro engineering of human autologous cartilage. A review |
Authors: | Daniela Bratosin, Catalin Iordachel, Alexandrina Rugina, Ana Maria Gheorghe, Andreea C.I. Ciotec , Nicolae Efimov, Manuela Sidoroff |
Affiliation: | 1 National Institute for Biological Science Research and Development, Bucharest, Romania 2 ”Vasile Goldis” Western University of Arad, Faculty of Biology, Arad, Romania 3 CFR 2 Hospital, Bucharest, Romania |
Abstract: | Articular cartilage repair remains a challenge for regenerative medicine. This review summarizes our research findings that have been undertaken, with a particular emphasis on those techniques that have been introduced into our laboratory, via in vitro and preclinical studies. |
Keywords: | osteoarthritis, chondrocytes, flow-cytometry, tissue engineering, regenerative medicine |
References: | Aigner T., Kim H.A., Apoptosis and cellular vitality: Issues in osteoarthritic cartilage degeneration, Arthritis Rheum., 46:1986-1996, 2002 Aigner T., Kim H.A., H. I. Roach, Apoptosis in osteoarthritis, Rheum. Dis. Clin. N. Am., 30:639-654, 2004 Almarza A.J., Athanasiou K.A., Effects of initial cell seeding density for the tissue engineering of the temporomandibular joint disc. Ann Biomed Eng., 33:943–950, 2005 Aydelotte M.B., Thonar E.J., Mollenhauer J., Flechtenmacher J., Culture of chondrocytes in alginate gel: variations in conditions of gelation influence the structure of the alginate gel, and the arrangement and morphology of proliferating chondrocytes. In Vitro Cell Dev Biol Anim., 34:123–130, 1998 Bratosin D., Estaquier J., Petit F., Arnoult D., Quatannens B., Tissier J.P., Slomianny C., Sartiaux C., Alonso C., Huart J.J., Ameisen J.C., Montreuil J., Programmed cell death in mature erythrocytes: a model for investigating death effector pathways operating in the absence of mitochondria, Cell Death Differ., 8:1143-1156, 2001 Bratosin D., Buia L., Heron A., Montreuil J., US Patent Application 20100256234, Method of stimulating proliferation of differentiated cells belonging to chondrocytes line”, Published on October 7, 2010 Buckwalter J.A., Lane N.E., Athletics and osteoarthritis. Am. J. Sports Med., 25, 873-881, 1997 Bursch W., Ellinger A., Gerner C., Frohwein U., R. Schulte-Hermann, Programmed cell death (PCD). Apoptosis, autophagic PCD or others ?, Ann. N.Y. Acad. Sci., 926:1-12, 2001 Buia-Takacs L., Iordachel C., Efimov N., Caloianu M., Montreuil J., Bratosin D., Flow cytometric analysis of encapsulated chondrocytes cultured in alginate gel for cartilage tissue engineering, Jurnal Medical Aradean (Arad Medical Journal), Vol. XIII, issue 4:25-33, 2010 DeLise A.M., Archer C.W., Benjamin M., Ralphs J.R., Organisation of the chondrocyte cytoskeleton and its response to changing mechanical conditions in organ culture, J. Anat., 194, 343-35, 1999 Fukuda T., Wang H., Nakanishi H., Kosaka K., Yamamoto T., Novel non-apoptotic morphological changes in neurons of the mouse hippocampus following transient hypoxic-ischemia, Neurosci. Res., 33:49-55, 1999 Gold G.E., Bergman A.G., Pauly J.M., Lang P., Butts R.K., Beaulieu C.F., Hargreaves B., Frank L., Boutin R.D., Macovski A., Resnick D., Magnetic resonance imaging of knee cartilage repair. Top Mag Reson Imag., 9, 377-392, 1998 Grandolfo M., D’andrea P., Paoletti S., Martina M., Silvestrini G., Bonucci E., Vittur F., Culture and differentiation of chondrocytes entrapped in alginate gels. Calcif Tissue Int., 52:42–48, 1993 Guo J.F., Jourdian G.W., Maccallum D.K., Culture and growth characteristics of chondrocytes encapsulated in alginate beads, Connect Tissue Res., 19:277–297, 1989 Hamilton D.W., Riehle M.O., Monaghan W., Curtis A.S., Articular chondrocyte passage number: Influence on adhesion, migration, cytoskeletal organisation and phenotype in response to nano- and micro-metric topography. Cell Biol Int., 29: 408–421, 2005 Kerr J.F.R., Wyllie A.H., Currie A.R., Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics, Br. J. Cancer, 26:239- 257,1972 Koch R.J., Gorti G.K., Tissue engineering with chondrocytes. Facial Plast Surg., 18:59–68, 2002 Leist M., Jaattela M., Four death and a funeral: From caspases to alternative mechanisms, Nat. Rev. Mol. Cell Biol., 2:589-598, 2001 Li Y., Ma T., Kniss D.A., Laski L.C., Yang S.T., Effects of filtration seeding on cell density, spatial distribution, and proliferation in nonwoven fibrous matrices. Biotechnol Prog., 17: 935–944, 2001 Liu X., Ma P.X., Polymeric scaffolds for bone tissue engineering. Ann Biomed Eng., 32: 477–486, 2004 Martin J.A., Buckwalter J.A., Aging, articular cartilage chondrocyte senescence and osteoarthritis, Biogerontolog,. 3, 257-264, 2002 Mcfetridge P.S., Bodamyali T., Horrocks M., Chaudhuri J.B., Endothelial and smooth muscle cell seeding onto processed ex vivo arterial scaffolds using 3D vascular bioreactors. ASAIO J., 50:591–600, 2004 Muir H., The chondrocyte, architect of cartilage. Biomechanics, structure, function and molecular biology of cartilage matrix macromolecules, Bioessays, 17, 1039-1048, 1995 Paige K.T., Cima L.G., Yaremchuk M.J., Vacanti J.P., Vacanti C.A., Injectable cartilage. Plast Reconstr Surg., 96:1390–1398, 1995 Price J.S., Waters J.G., Darrah C., Pennington C., Edwards D.R., Donell S.T., Clark I.M., The role of chondrocyte senescence in osteoarthritis, 1, 57-65, 2002 Radisic M., Euloth M., Yang L., Langer L.E., Freed G. Vunjak-Novakovic, High-density seeding of myocyte cells for cardiac tissue engineering. Biotechnol Bioeng., 82: 403–414, 2003 Randolph M.A., Anseth K., Yaremchuk M.J., Tissue engineering of cartilage. Clin Plast Surg., 30:519– 537, 2003 Roach H.I., Clarke N.M.P., Cell paralysis as an intermediate stage in the programmed cell death of epiphyseal chondrocytes, J.Bone Miner Res, 14:1367-1378, 1999 Roach H.I., Clarke N.M.P., Physiological cell death of chondrocytes in vivo is not confined to apoptosis: New observations on the mammalian growth plate, J. Bone Joint Surg., 82-B:601- 613, 2001 Roach H.I., Aigner T., Kouri J.B., Chondroptosis: A variant of apoptotic cell death in chondrocytes ?, Apoptosis, 9:265-27, 2004 Saxon L., Finch C., Bass S., Sports participation, sports injuries and osteoarthritis: Implications for prevention. Sports Med., 28, 123-135, 1999 Shimizu K., Ito A., Honda H., Enhanced cell-seeding into 3D porous scaffolds by use of magnetite nanoparticles. J Biomed Mater Res B Appl Biomater, 77: 265–272, 2006 Soletti L., Nieponice A., Guan J., Stankus J.J., Wagner W.R., Vorp D.A., A seeding device for tissue engineered tubular structures. Biomaterials, 27: 4863–4870, 2006 Sperandio, De Belle I., Bredesen D.E., An alternative, nonapoptotic form of programmed cell death., Proc. Natl. Acad. Sci. USA, 97:14376-14381, 2000 Takacs-Buia L., Iordachel C., Efimov N., Caloianu M., Montreuil J., Bratosin D., Pathogenesis of osteoarthritis: Chondrocyte replicative senescence or apoptosis?, Cytometry Part B (Clinical Cytometry), 74B:356–362,2008 Ushida T., Furukawa K., Toita K., Tateishi T., Threedimensional seeding of chondrocytes encapsulated in collagen gel into PLLA scaffolds. Cell Transplant, 11: 489–494, 2002 Vignon E., Arlot M., Patricot I. M., Vignon G., The cell density of human femoral head cartilage, Clin. Orthop., 121:303-308, 1976 Vujak-Novakovic G., Radisic M., Cell seeding of polymer scaffolds. Methods Mol Biol., 238:131–146, 2004 Yang K.G., Saris D.B., Geuze R.E., Van Rijen M.H., Van Der Helm Y.J., Verbout A.J., Creemers L.B., Dhert W.J., Altered in vitro chondrogenic properties of chondrocytes harvested from unaffected cartilage in osteoarthritic joints. Osteoarthritis Cartilage, 14: 561–570, 2006 Yudoh K., Nguyen T., Nakamura H., Hongo-Masuko K., Kato T., Nishioka K., Potential involvement of oxidative stress in cartilage senescence and development of osteoarthritis: oxidative stress induces chondrocyte telomere instability and downregulation of chondrocyte function, Arthritis Res. Ther., 7, 380-391, 2005 Zhao F., Ma T., Perfusion bioreactor system for human mesenchymal stem cell tissue engineering: Dynamic cell seeding and construct development. Biotechnol Bioeng., 91:482–493, 2005 |
*Correspondence: |