Exposure of HELA cells to low dose of single walled carbon nanotubes functionalized with single strand DNA induces no signifficant toxicity

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Article Title:	Exposure of HELA cells to low dose of single walled carbon nanotubes functionalized with single strand DNA induces no signifficant toxicity
Authors:	Teodora Mocan, Cornel Iancu, Ariana Stir, Ştefania Simon, Alexandru Radu Biris, Lucian Mocan, Simona Clichici, Adriana Muresan
Affiliation:	1 Department of Physiology, University of Medicine and Pharmacy “Iuliu Hatieganu” Cluj-Napoca, Romania 2 Nanomedicine Department, Institute of Gastroenterology and Hepatology, Cluj-Napoca, Romania 3 Hydrogen Department, National Institute for Molecular and Isotopic Technologies, Cluj-Napoca, Romania.
Abstract:	Introduction. Single-walled carbon nanotubes(SWCNT) represents a promising material for the biomedical field. However, reports have appeared that suggest that toxic effects can appear following SWCNT exposure. Aim. Present study aims to evaluate the in vitro effects following exposure to low dose of single walled carbon nanotubes functionalized with single strand DNA (SWCNT-ss-DNA) over HeLa cells. Material and Method. Synthesis of the SWCNT (inductive heating method, acetylene, 850oC, Fe:Mo:MgO catalyst), followed by non-covalent binding of single strand DNA(ss-DNA) through sonication. HeLa cell line was exposed to 1mg/L ss-DNA-SWCNT solution with monitoring at 15 min, 24 hrs, 48 hrs. Results. No toxic effects were observed at microscopic evaluation of cell morphology and at quantitative viability evaluation by means of MTT assay.(p>0.05). Conclusions. Limited dose exposure of HeLa cells to SWCNT-ss-DNA induces no cytotoxic effects.
Keywords:	carbon nanotubes, DNA, cytotoxicity, low concentration, in vitro
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*Correspondence:	University of Medicine and Pharmacy “Iuliu Hatieganu” Cluj-Napoca Physiology Department, Clinicilor 1, 40006 Cluj-Napoca, Romania; Phone: +40-744-345310; Fax +40-264-597257; Email: teodora_mocan@yahoo.com