Stability of effector protein cagA: a prerequisite for HP physiology and pathology

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Article Title:	Stability of effector protein cagA: a prerequisite for HP physiology and pathology
Authors:	Ioana Lancrajan
Affiliation:	„Vasile Goldiş” Western University of Arad, Faculty of Life Sciences, Romania
Abstract:	Helicobacter pylori colonizes the human stomach and can induce gastritis, peptic ulceration, gastric MALT lymphoma and gastric adenocarcinoma, the second most common cause of cancer mortality in the world. We suppose that the role of effector protein CagA in pathology of HP infection is partially influenced by a very diverse and extreme environment in the stomach, with consequences on the structure and functionality of CagA and hence on physiopatology of HP. The purpose of this study is to evaluate structural and functional CagA, the single known effector protein of Helicobacter pylori under physiological conditions.Our result indicate CagA as a very susceptible molecule to degradation dependent on medium factors. Recombinant CagA shown a very high instability and degradation under different physical-chemical factors, selective degradation especially at the functional significantly C-terminus, high sensitivity and degradation by mechanical stress and high temperature, higher temperature favouring faster degradation as lower temperature.
Keywords:	stability, effector protein, environment, degradation, stomach
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*Correspondence:	Ioana Lancrajan, „Vasile Goldiş” Western University of Arad, Faculty of Life Sciences, Email: Lancrajan _ioana@yahoo.com