Determination of mineral contents and antioxidant activity in some plants that contain allelochemicals of Banat region (western Romania)

September 6, 2012

Determination of mineral contents and antioxidant activity in some plants that contain allelochemicals of Banat region (western Romania)

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Title:	Determination of mineral contents and antioxidant activity in some plants that contain allelochemicals of Banat region (western Romania)
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Article_Title:	Determination of mineral contents and antioxidant activity in some plants that contain allelochemicals of Banat region (western Romania)
Authors:	Monica Butnariu, Cristian Bostan, Ionel Samfira
Affiliation:	Chemistry and Vegetal Biochemistry, Banat’s University of Agricultural Sciences and Veterinary Medicine, Timisoara, Romania, Department of Grasslands and Forage Crops, Banat’s University of Agricultural Sciences and Veterinary Medicine, Timisoara, Romania, Department of Grasslands and Forage Crops, Banat’s University of Agricultural Sciences and Veterinary Medicine, Timisoara, Romania,
Abstract:	This study was designated to evaluate the correlation between total polyphenols and mineral content with antioxidant activity in various extracts of plants that contain allelochemicals Banat region. The study was carried out to analyze the mineral contents and antioxidant activity of the leaves of Setaria viridis, Datura stramonium Sorghum halepense and Aristolochia clematitis L. collected from different Banat zones, using standard method of plants analysis. Among the various macronutrients estimated in the plant samples of different wild leaves plant potassium was present in the highest quantity (16.10–84.40 mg g–1) followed by calcium (5.85–23.70 mg g–1) and sodium (0.95–3.20 mg g–1). Metals, such as iron, zinc, copper, manganese and chromium were analyzed in the different plants. The antioxidant activity of leaves plants was examined. Phenols content of the extract were measured by FC and AlCl3 assays. The content of total polyphenols in the aqueous methanolic extracts was calculated as GAE and radical scavenging activity was estimated as IC50 values using 1, 1–diphenyl–2–picrylhydrazyl (DPPH). The total phenols varied from 17.07±0.13 to 67.26±0.24 mg g–1 in the extracts. The highest radical scavenging effect was observed in Aristolochia clematitis L. with IC50 = 141.04±0.25 μg mL–1. The greater amount of polyphenolic compound leads to more potent radical scavenging effect as shown by Aristolochia clematitis L. The methanol extracts of these plants exhibited significant antioxidant activities by different assays and contained significant levels of polyphenols. These results show that methanolic extracts of these plants that contain allelochemicals, could be considered as a natural alternative source for drugs,pharmacology and medicine sectors.
Keywords:	metals, leaves plants, antioxidant activity, phenol.
References:	Sahito SR, Memon MA, Kazi TG, Kazi GH, Evaluation of mineral contents in medicinal plant Azadirachta indica (Neem), J. Chem. Soc. Pakistan. 2003. 25. 139–143. Audu AA, Lawal AO. Variation in Metal Contents of Plants in Vegetable Garden Sites in Kano Metropolis, J. Appl. Sci. Environ. Mgt. 2006, 10 (2) 105–109. Jabeen S, Shah MT, Khan S, Hayat MQ. Determination of major and trace elements in ten important folk therapeutic plants of Haripur basin, Pakistan, J. Med. Plants Res. 2010. 4:559–566. Essiett UA, Effiong GS, Akang VE, Trace metal Contents in Medicinal Plants Growing in Crude Oil Polluted Soil in Akwa Ibom State, Southeastern Nigeria, Archives of Applied Science Research, 2011, 3 (1):9–16. Khan SA, Khan L, Hussain I, Marwat KB, Akhtar N. Profile of heavy metals in selected medicinal plants. Pak. J. Weed Sci. Res. 2008. 14:101–110. Sharma KR, Agrawal M, Marshall MF. Heavy metals in vegetables collected from production and market sites of a tropical urban area of India, Food Chem. Toxicol. 2009. 47:583–591. Basgel S, Erdemoglu SB. Determination of mineral and trace elements in some medicinal herbs and their infusions consumed in Turkey, Sci. Total Environ. 2006. 359:82–89. Karimi G, Hasanzadeh MK, Nili A, Khashayarmanesh Z, Samiei Z, Nazari F, Teimuri M. Concentrations and health risk of heavy metals in tea samples marketed in Iran, Pharmacology 2008. 3:164–174. Nookabkaew S, Rangkadilok N, Satayavivad J. Determination of trace elements in herbal tea products and their infusions consumed in Thailand J. Agr. Food Chem. 2006. 54:6939–6944. Lasheen YF, Awwad NS, El–khalafawy A, Abdel–Rassoul AA. Annual effective dose and concentration levels of heavy metals in different types of tea in Egypt, Int. J. Phys. Sci. 2008. 3:112–118. El-Khatib AA. The response of some common Egyptian plants ozone and their use a biomonitors. Environmental Pollution. 2003. 124: 419–428. Saeedeh AD, Urooj A. Antioxidant properties of various solvent extracts of mulberry (Morus indica L.) leaves. Food Chem. 2007. 102:1233–1240. Xiaoli L, Mouming Z, Jinshui W, Bao Y, Yueming J. Antioxidant activity of methanol extracts of emblica fruit (Phyllanthus emblica L) from six regions in china. J. Food Compos. Anal. 2008. 21:219–228. Zhou S, Fang Z, Lu Y, Chen J, Liu D, Ye X. Polyphenols and antioxidant properties of bayberry (Myrica rubra Sieb. et Zucc.) pomace. Food Chem. 2009. 112(2):394–399. Rodino S, Buţu M, Golea D, Buţu A. Qualitative evaluation of fresh marc–raw material with valuable composition. Banat’s Journal of Biotechnology. 2011. 4(2):18–27. Park Y, Jung S, Kang S, Licon DE, Ayala A, Tapia MS, Belloso MO, Trakhtenberg S, Gorinstein S. Drying of persimmons (Diospyros kaki L.) and following changes in the studied bioactive compounds and the total radical scavenging activities. LWT–Food Sci. Technol. 2006. 39:748–755. Miliauskas G, Venskutonis PR, Van Beek TA. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chem. 2004. 85:231–237. Loukopoulos D. Combined therapy with deferiprone and desferrioxamine in thalassemia major.. Hematol. J. 2005. 90:1305–1305. Demiray S, Pintado ME, Castro PML. Evaluation of polyphenolic profiles and antioxidant activities of Turkish medicinal plants: Tiliaargentea, Crataegi folium leaves and Polygonum bistorta roots. World Acad. Sci. Eng. Technol. 2009. 54:312–317. Ebrahimzadeh MA, Pourmorad F, Hafezi S. Antioxidant Activities of Iranian Corn Silk. Turk. J. Biol. 2008b. 32: 43–49. A.O.A.C. Official Methods of Analysis, Association of Official Analytical Chemist, EUA. 2000. WHO, Quality Control Methods for Medicinal Plant Materials, Revised, Geneva, 2005. ISO–14502–1:2005. AOAC 941.15.AOAC, 2003. Chen Z, Newman I, Zhou M, Mendham N, Zhang G, Shabala S. Screening plants for salt tolerance by measuring K+ flux: a case study for barley. Plant Cell Environ. 2005. 28:1230–1246. Laladhas KP, Cheriyan VT, Puliappadamba VT, Bava SV, Unnithan RG, Vijayammal PL, Anto RJ. A novel protein fraction shows Potential anticancer and chemopreventive efficacy, in vitro and in vivo. J Cell Mol Med. 2010. 14(3):636–646. Boonmee C, Reynolds D, Sangvanich P. Alphaglucosidase inhibitor proteins from Sesbania grandiflora flowers. Planta Med. 2007. 73(11):1197–201. Kretovich UL. Principles of plant biochemistry permagon, Oxford Press J. Food Sci. 2005. 54: 254–260. Bucic–Kojic A, Planinic M, Tomas S, Jakobek L, Seruga M. Influence of solvent and temperature on extraction of polyphenolic compounds from grape seed, antioxidant activity and colour of extract. Intl. J. Food Sci. Technol. 2009. 44: 2394–2401. Akowuah GA, Ismail Z, Norhayati I, Sadikum A. The effect of different extraction solvents of varying polarities on polyphenols of Orthosiphon stamineus and evaluation of the free radical–scavenging activity. Food Chem. 2005. 93: 311–317.
Read_full_article:	pdf/22-2012/22-1-2012/SU22-1-2012-Butnariu.pdf
Correspondence:	monicabutnariu@yahoo.com, bostancristian2000@yahoo.com, samfiraionel@yahoo.ro

	Read full article
Article Title:	Determination of mineral contents and antioxidant activity in some plants that contain allelochemicals of Banat region (western Romania)
Authors:	Monica Butnariu, Cristian Bostan, Ionel Samfira
Affiliation:	Chemistry and Vegetal Biochemistry, Banat’s University of Agricultural Sciences and Veterinary Medicine, Timisoara, Romania, Department of Grasslands and Forage Crops, Banat’s University of Agricultural Sciences and Veterinary Medicine, Timisoara, Romania, Department of Grasslands and Forage Crops, Banat’s University of Agricultural Sciences and Veterinary Medicine, Timisoara, Romania,
Abstract:	This study was designated to evaluate the correlation between total polyphenols and mineral content with antioxidant activity in various extracts of plants that contain allelochemicals Banat region. The study was carried out to analyze the mineral contents and antioxidant activity of the leaves of Setaria viridis, Datura stramonium Sorghum halepense and Aristolochia clematitis L. collected from different Banat zones, using standard method of plants analysis. Among the various macronutrients estimated in the plant samples of different wild leaves plant potassium was present in the highest quantity (16.10–84.40 mg g–1) followed by calcium (5.85–23.70 mg g–1) and sodium (0.95–3.20 mg g–1). Metals, such as iron, zinc, copper, manganese and chromium were analyzed in the different plants. The antioxidant activity of leaves plants was examined. Phenols content of the extract were measured by FC and AlCl3 assays. The content of total polyphenols in the aqueous methanolic extracts was calculated as GAE and radical scavenging activity was estimated as IC50 values using 1, 1–diphenyl–2–picrylhydrazyl (DPPH). The total phenols varied from 17.07±0.13 to 67.26±0.24 mg g–1 in the extracts. The highest radical scavenging effect was observed in Aristolochia clematitis L. with IC50 = 141.04±0.25 μg mL–1. The greater amount of polyphenolic compound leads to more potent radical scavenging effect as shown by Aristolochia clematitis L. The methanol extracts of these plants exhibited significant antioxidant activities by different assays and contained significant levels of polyphenols. These results show that methanolic extracts of these plants that contain allelochemicals, could be considered as a natural alternative source for drugs,pharmacology and medicine sectors.
Keywords:	metals, leaves plants, antioxidant activity, phenol.
References:	Sahito SR, Memon MA, Kazi TG, Kazi GH, Evaluation of mineral contents in medicinal plant Azadirachta indica (Neem), J. Chem. Soc. Pakistan. 2003. 25. 139–143. Audu AA, Lawal AO. Variation in Metal Contents of Plants in Vegetable Garden Sites in Kano Metropolis, J. Appl. Sci. Environ. Mgt. 2006, 10 (2) 105–109. Jabeen S, Shah MT, Khan S, Hayat MQ. Determination of major and trace elements in ten important folk therapeutic plants of Haripur basin, Pakistan, J. Med. Plants Res. 2010. 4:559–566. Essiett UA, Effiong GS, Akang VE, Trace metal Contents in Medicinal Plants Growing in Crude Oil Polluted Soil in Akwa Ibom State, Southeastern Nigeria, Archives of Applied Science Research, 2011, 3 (1):9–16. Khan SA, Khan L, Hussain I, Marwat KB, Akhtar N. Profile of heavy metals in selected medicinal plants. Pak. J. Weed Sci. Res. 2008. 14:101–110. Sharma KR, Agrawal M, Marshall MF. Heavy metals in vegetables collected from production and market sites of a tropical urban area of India, Food Chem. Toxicol. 2009. 47:583–591. Basgel S, Erdemoglu SB. Determination of mineral and trace elements in some medicinal herbs and their infusions consumed in Turkey, Sci. Total Environ. 2006. 359:82–89. Karimi G, Hasanzadeh MK, Nili A, Khashayarmanesh Z, Samiei Z, Nazari F, Teimuri M. Concentrations and health risk of heavy metals in tea samples marketed in Iran, Pharmacology 2008. 3:164–174. Nookabkaew S, Rangkadilok N, Satayavivad J. Determination of trace elements in herbal tea products and their infusions consumed in Thailand J. Agr. Food Chem. 2006. 54:6939–6944. Lasheen YF, Awwad NS, El–khalafawy A, Abdel–Rassoul AA. Annual effective dose and concentration levels of heavy metals in different types of tea in Egypt, Int. J. Phys. Sci. 2008. 3:112–118. El-Khatib AA. The response of some common Egyptian plants ozone and their use a biomonitors. Environmental Pollution. 2003. 124: 419–428. Saeedeh AD, Urooj A. Antioxidant properties of various solvent extracts of mulberry (Morus indica L.) leaves. Food Chem. 2007. 102:1233–1240. Xiaoli L, Mouming Z, Jinshui W, Bao Y, Yueming J. Antioxidant activity of methanol extracts of emblica fruit (Phyllanthus emblica L) from six regions in china. J. Food Compos. Anal. 2008. 21:219–228. Zhou S, Fang Z, Lu Y, Chen J, Liu D, Ye X. Polyphenols and antioxidant properties of bayberry (Myrica rubra Sieb. et Zucc.) pomace. Food Chem. 2009. 112(2):394–399. Rodino S, Buţu M, Golea D, Buţu A. Qualitative evaluation of fresh marc–raw material with valuable composition. Banat’s Journal of Biotechnology. 2011. 4(2):18–27. Park Y, Jung S, Kang S, Licon DE, Ayala A, Tapia MS, Belloso MO, Trakhtenberg S, Gorinstein S. Drying of persimmons (Diospyros kaki L.) and following changes in the studied bioactive compounds and the total radical scavenging activities. LWT–Food Sci. Technol. 2006. 39:748–755. Miliauskas G, Venskutonis PR, Van Beek TA. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chem. 2004. 85:231–237. Loukopoulos D. Combined therapy with deferiprone and desferrioxamine in thalassemia major.. Hematol. J. 2005. 90:1305–1305. Demiray S, Pintado ME, Castro PML. Evaluation of polyphenolic profiles and antioxidant activities of Turkish medicinal plants: Tiliaargentea, Crataegi folium leaves and Polygonum bistorta roots. World Acad. Sci. Eng. Technol. 2009. 54:312–317. Ebrahimzadeh MA, Pourmorad F, Hafezi S. Antioxidant Activities of Iranian Corn Silk. Turk. J. Biol. 2008b. 32: 43–49. A.O.A.C. Official Methods of Analysis, Association of Official Analytical Chemist, EUA. 2000. WHO, Quality Control Methods for Medicinal Plant Materials, Revised, Geneva, 2005. ISO–14502–1:2005. AOAC 941.15.AOAC, 2003. Chen Z, Newman I, Zhou M, Mendham N, Zhang G, Shabala S. Screening plants for salt tolerance by measuring K+ flux: a case study for barley. Plant Cell Environ. 2005. 28:1230–1246. Laladhas KP, Cheriyan VT, Puliappadamba VT, Bava SV, Unnithan RG, Vijayammal PL, Anto RJ. A novel protein fraction shows Potential anticancer and chemopreventive efficacy, in vitro and in vivo. J Cell Mol Med. 2010. 14(3):636–646. Boonmee C, Reynolds D, Sangvanich P. Alphaglucosidase inhibitor proteins from Sesbania grandiflora flowers. Planta Med. 2007. 73(11):1197–201. Kretovich UL. Principles of plant biochemistry permagon, Oxford Press J. Food Sci. 2005. 54: 254–260. Bucic–Kojic A, Planinic M, Tomas S, Jakobek L, Seruga M. Influence of solvent and temperature on extraction of polyphenolic compounds from grape seed, antioxidant activity and colour of extract. Intl. J. Food Sci. Technol. 2009. 44: 2394–2401. Akowuah GA, Ismail Z, Norhayati I, Sadikum A. The effect of different extraction solvents of varying polarities on polyphenols of Orthosiphon stamineus and evaluation of the free radical–scavenging activity. Food Chem. 2005. 93: 311–317.
*Correspondence:	monicabutnariu@yahoo.com, bostancristian2000@yahoo.com, samfiraionel@yahoo.ro

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