Determination of assimilator pigments content in cotyledons of pinus nigra arn. Plantlets illuminated with light-emitting diodes (LEDs)

Determination of assimilator pigments content in cotyledons of pinus nigra arn. Plantlets illuminated with light-emitting diodes (LEDs)

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Title: Determination of assimilator pigments content in cotyledons of pinus nigra arn. Plantlets illuminated with light-emitting diodes (LEDs)
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Article_Title: Determination of assimilator pigments content in cotyledons of pinus nigra arn. Plantlets illuminated with light-emitting diodes (LEDs)
Authors: Mirela Maria Matioc-Precup, Dorina Cachiţă-Cosma
Affiliation: Faculty of Sciences, University of Oradea, Oradea Faculty of Natural Sciences,”Vasile Goldiş” Western University of Arad, Arad
Abstract: The experiment focused on determining the assimilator pigments content in the cotyledons of the black pine plantlets (Pinus nigra Arn.) derived from seeds germinated and grown for 21 days, 16 hours/day, under light emitted by Light-Emitting Diodes (LEDs), of different colours (respectively, of different wavelengths). The light intensity was constant, of 1200 lx. The highest chlorophyll a content was recorded at the seedlings exposed to the red light produced by LEDs, which, compared with the plantlets grown in natural light, marked an increase of 26.3%, and, as against to those exposed to fluorescent white light, was 18.1% higher. Instead, the seedlings exposed to white LEDs light, in relation to those illuminated with natural light, have registered the highest values of chlorophyll b (+159%) and carotenoid pigments (+95.2%), and of the total chlorophyll pigments content (+74.7%), respectively, of the total assimilator pigments (+77.1%). The lowest values of the assimilator pigments content were marked at the plantlets exposed to the green light of LEDs, at which the chlorophyll a content was reduced with 25.7%, and the content in chlorophyll b with 31.3%, in comparison with the results noted at the group of plantlets lighted with white fluorescent tubes; therefore, the total green pigments were diminished with 28.9%, respectively, the content in total asimilator pigments with 28.8%. Thus, from all the types of lightings used, the most effective light for the assimilator pigments accumulation, in the cotyledons of black pine seedlings, proved to be the white one of LEDs
Keywords: assimilator pigments, Pinus nigra, cotyledons, Light-Emitting Diodes, fluorescent light
References: Bula R.J., Tennessen D.J., Morrow R.C., Tibbitts T.W., (1994) – Light emitting diodes as a plant lighting source. T.W.Tibbitts (ed.), International Lighting in Controlled Environments Workshop, pp. 255-267.
Kim H.H., Goins G.D., Wheeler R.M., Sager J.C., (2004) – Green-light supplementation for enhanced lettuce growth under Red- and Blue-light-emitting Diodes. HortScience, Vol. 39, Nr. 7, pp. 1617-1622.
Matioc-Precup M.M., Cachiţă-Cosma D., (2011) – Effects of light of different wavelengths, emitted by Light-Emitting Diodes (LEDs) on the germination of seeds of Pinus nigra Arn. and on the growth of the plantlets resulted from their embryos. Studia Universitatis “Vasile Goldiş”, Life Sciences Series, Vol. 21, Nr. 3, pp. 625-632.
Moran R., Porath D., (1980) – Chlorophyll determination in intact tissues using N,N-dimetylformamide. Plant Physiology, Vol. 65, pp. 478 – 479.
Tikhomirov A.A., (1994) – Spectral composition of light and growing of plants in controlled environments. T.W.Tibbitts (ed.), International Lighting in Controlled Environments Workshop, pp. 25-29.
Urbonaviciute A., Pinho P., Samuoliene G, Duchovskis P., Vitta P., Stonkus A., Tamulaitis G., Zukauskas A., Halonen L., (2007) – Effect of short-wavelength light on lettuce growth and nutritional quality. Scientific Works of the Lithuanian Institute of Horticulture and Lithuanian University of Agriculture, Vol. 26, Nr. 1, pp. 157-165.
Vidican T.I., Cachiţă C.D., (2010) – Determination of assimilator pigment content in cladodes of Opuntia fragilis var. fragilis exposed to light of different colors emitted by LEDs. Studia Universitatis “Vasile Goldiş”, Life Sciences Series, Vol. 20, Nr. 2, pp. 49-54.
Wellburn A.R., (1994) – The spectral determination of chlorophylls a and b, as well as total carotenoides, using various solvents with spectrophotometers of different resolution. Plant Physiology, Vol. 144, Nr. 1, pp. 307 – 313.
Yorio N.C., Goins G.D., Kagie H.R., Wheeler R.M., Sager J.C., (2001) – Improving spinach, radish, and lettuce growth under red Light-emitting Diodes (LEDs) with blue light supplementation. HortScience, Vol. 36, Nr. 2, pp. 380-383.
Read_full_article: pdf/22-2012/22-1-2012/SU22-1-2012-Matioc.pdf
Correspondence: mirelamatioc@yahoo.com

Read full article
Article Title: Determination of assimilator pigments content in cotyledons of pinus nigra arn. Plantlets illuminated with light-emitting diodes (LEDs)
Authors: Mirela Maria Matioc-Precup, Dorina Cachiţă-Cosma
Affiliation: Faculty of Sciences, University of Oradea, Oradea Faculty of Natural Sciences,”Vasile Goldiş” Western University of Arad, Arad
Abstract: The experiment focused on determining the assimilator pigments content in the cotyledons of the black pine plantlets (Pinus nigra Arn.) derived from seeds germinated and grown for 21 days, 16 hours/day, under light emitted by Light-Emitting Diodes (LEDs), of different colours (respectively, of different wavelengths). The light intensity was constant, of 1200 lx. The highest chlorophyll a content was recorded at the seedlings exposed to the red light produced by LEDs, which, compared with the plantlets grown in natural light, marked an increase of 26.3%, and, as against to those exposed to fluorescent white light, was 18.1% higher. Instead, the seedlings exposed to white LEDs light, in relation to those illuminated with natural light, have registered the highest values of chlorophyll b (+159%) and carotenoid pigments (+95.2%), and of the total chlorophyll pigments content (+74.7%), respectively, of the total assimilator pigments (+77.1%). The lowest values of the assimilator pigments content were marked at the plantlets exposed to the green light of LEDs, at which the chlorophyll a content was reduced with 25.7%, and the content in chlorophyll b with 31.3%, in comparison with the results noted at the group of plantlets lighted with white fluorescent tubes; therefore, the total green pigments were diminished with 28.9%, respectively, the content in total asimilator pigments with 28.8%. Thus, from all the types of lightings used, the most effective light for the assimilator pigments accumulation, in the cotyledons of black pine seedlings, proved to be the white one of LEDs
Keywords: assimilator pigments, Pinus nigra, cotyledons, Light-Emitting Diodes, fluorescent light
References: Bula R.J., Tennessen D.J., Morrow R.C., Tibbitts T.W., (1994) – Light emitting diodes as a plant lighting source. T.W.Tibbitts (ed.), International Lighting in Controlled Environments Workshop, pp. 255-267.
Kim H.H., Goins G.D., Wheeler R.M., Sager J.C., (2004) – Green-light supplementation for enhanced lettuce growth under Red- and Blue-light-emitting Diodes. HortScience, Vol. 39, Nr. 7, pp. 1617-1622.
Matioc-Precup M.M., Cachiţă-Cosma D., (2011) – Effects of light of different wavelengths, emitted by Light-Emitting Diodes (LEDs) on the germination of seeds of Pinus nigra Arn. and on the growth of the plantlets resulted from their embryos. Studia Universitatis “Vasile Goldiş”, Life Sciences Series, Vol. 21, Nr. 3, pp. 625-632.
Moran R., Porath D., (1980) – Chlorophyll determination in intact tissues using N,N-dimetylformamide. Plant Physiology, Vol. 65, pp. 478 – 479.
Tikhomirov A.A., (1994) – Spectral composition of light and growing of plants in controlled environments. T.W.Tibbitts (ed.), International Lighting in Controlled Environments Workshop, pp. 25-29.
Urbonaviciute A., Pinho P., Samuoliene G, Duchovskis P., Vitta P., Stonkus A., Tamulaitis G., Zukauskas A., Halonen L., (2007) – Effect of short-wavelength light on lettuce growth and nutritional quality. Scientific Works of the Lithuanian Institute of Horticulture and Lithuanian University of Agriculture, Vol. 26, Nr. 1, pp. 157-165.
Vidican T.I., Cachiţă C.D., (2010) – Determination of assimilator pigment content in cladodes of Opuntia fragilis var. fragilis exposed to light of different colors emitted by LEDs. Studia Universitatis “Vasile Goldiş”, Life Sciences Series, Vol. 20, Nr. 2, pp. 49-54.
Wellburn A.R., (1994) – The spectral determination of chlorophylls a and b, as well as total carotenoides, using various solvents with spectrophotometers of different resolution. Plant Physiology, Vol. 144, Nr. 1, pp. 307 – 313.
Yorio N.C., Goins G.D., Kagie H.R., Wheeler R.M., Sager J.C., (2001) – Improving spinach, radish, and lettuce growth under red Light-emitting Diodes (LEDs) with blue light supplementation. HortScience, Vol. 36, Nr. 2, pp. 380-383.
*Correspondence: mirelamatioc@yahoo.com