Effect of pollution in the flora , microflora and soil enzyme activities near to the Upper-Tisza

Effect of pollution in the flora , microflora and soil enzyme activities near to the Upper-Tisza

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Title: Effect of pollution in the flora , microflora and soil enzyme activities near to the Upper-Tisza
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Article_Title: Effect of pollution in the flora , microflora and soil enzyme activities near to the Upper-Tisza
Authors: Judit L. Halász1*, Zsolt Kotroczo1, Erzsebet Krausz2, Márta D. Tóth1, Sándor Balázsy1
Affiliation: ¹ Institute of Biology, College of Nyíregyháza, Nyíregyháza, Hungary
2Department of Environmental Science, College of Nyíregyháza, Nyíregyháza, Hungary
Abstract: Our main purpose was to study the flora, the microflora and the enzyme activity in soil of polluted areas, and to identify the factors affecting them. Besides studying the percentage distribution of species in the soil, we determined the total number of culturable microbes as well. Enzyme activities of phosphatase, invertase, dehydrogenase, and cellulase, were also measured in the samples of the contaminated soils. Total heavy metal content in soil samples
was determined by analysis of Cd, Cu, Fe, Pb and Zn using XRF technique. According to our examinations, ratio of Actinomyces and Pseudomonas genera decreased while the Bacillus sp. was found to be increased in the soils. Wastes reduce the activity of soil enzymes (cellulase, phosphatase, invertase), however, the activity of dehydrogenase increase.
Keywords: enzyme activity, soil microorganisms, plants, contaminated soil
References: Bartholomev JW, Mittwer T, A simlified bacterial spore stain. Stain Tech, 25, 153-154, 1950.
Borie F, Fuenteacba R, Biochemistry of volcanic ash soils:2. Urease activity. Agricultura-Technica, 42, 135-142, 1982.
Chander K, Brookes P, Microbial biomass dynamics during the decomposition of glucose and maize in metal-contaminated and not-contaminated soils. Soil Biology and Biochemistry (Oxford), 23, 917- 925, 1991.
Dick, WA, Tabatabai MA, Significance and potential uses of soil enzymes. In: Blain, F.J. (Ed.), Soil Microbial Ecology Application in Agricultural and Environmental Management. New York: Marcel Dekker, 95-127, 1992.
Frankenberger WT, Johanson JB, Method of measuring invertase activity in soils. Plant and Soil, 74, 301-311, 1983.
Garcia C, Hernandez T, Costa F, Potential use of dehydrogenase activity as an index of microbial activity in degraded soils. Communications in soil science and plant analysis, 28, 123-134, 1997.
Hattori H, Influence of cadmium on decomposition of glucose and cellulose in soil. Soil Science and Plant Nutrition (Tokyo), 37, 39-45, 1991.
Hucker GJ, Comparison of various methods of Gram staining. Abstr. Bact, 6, 2-8, 1922.
Hugh R, Leifson FI, The taxonomic significance of fermentative versus oxidative metabolism of carbohydrate, by various Gram negative bacteria. Journal of Bacteriologie, 66, 21-28, 1953.
Kovács G, Catalase Activity- an Indicator of Biological Activity in Forest Soil. Agrokémia és Talajtan, 39, 439-443, 1990.
Krámer M, Erdei G, Application of the method of phosphatase activity determination in agricultural chemistry. Soviet Soil Sci, 9, 1100-1103, 1959.
Kuprevics VF, Scserbakova TA, Csjupa GP, Soil urease activity. Dokl. Akad. Navuk. Belarusk, SSR 10, 336-338, 1966.
Lee IS, Kim OK, Chang YY, Bae B, Kim HH, Baek HK, Heavy metal concentracions and enzyme activities in soil from a contaminated Korean shooting range. Journal of Bioscience and Bioengineering, 94, 406- 411, 2002.
Mersi von W, Scinner F, An improved and accurate method for determining the dehydrogenase activity of soils with iodonitrotetrazolium chlorid. Biology and Fertility of Soils, 11, 216-220. 1991.
Moller H, Saccharase and urease content of peat and muick soils in north-west German aldercommunities, 9, 175-192, 1979.
Pascual JA, Moreno JL, Hernández T, Garcia C, Persistence of immobilised and total urease and phosphatase activities in a soil amended with organic wastes. Bioresource Technology, 82, 73-78, 2002.
Pascual I, Antolín MC, Garcia C, Polo A, Díaz MS, Effect of water deficit on microbial characteristics in soil amended with sewage sludge or inorganic fertilizer under laboratory conditions. Bioresource Technology, 98, 29-37, 2007.
Poll C, Thiede A, Wermbter N, Sessitsch A, Kandeler E, Micro-scale distribution of microorganisms and microbial enzyme activities in a soil with longterm organic amendment. European journal of soil science, 54, 715-724, 2003.
Reddy RU, Reddy MS, Jayakumar GWL, Urease activity in soils of northern Telanguna of Andhra pradesh, and its relationship with various soil properties. Annuals of Agricultural Research, 17, 261-264, 1996.
Smith LJ, Papendick RI, Soil organic matter dynamics and crop residue management. In: Metting, B. (Ed9, Soil Microbial Ecology. Marcell Dekker, New York, USA. 1993.
Tabatabai MA, Soil enzymes. In: Micelson, S. H., Bigham, J. E. (Eds.), Methods of Soil Analysis. Part 2: Microbial and Biochemical Properties. Madison: Soil Science Society of America, 775-826, 1994.
Thirukkumaran CM, Parkinson D, Microbial respiration, biomass, metabolic quotient and litter decomposition in a lodgepole pine forest floor amended with nitrogen and phosphorus fertilizer. Soil Biology and Biochemistry, 32, 59-66, 2000.
Unger-féle cellulózteszt módszer a talajbiológiai aktivitás meghatározására. In: Szegi. Talajmikrobiológiai vizsgálati módszerek. 245-247. Mezőgazdasági Kiadó. Budapest. 1979.
Zhang CB, Zhang ZH, A study of dynamic relationship between soil organic matter and enzymatic activity. Soils and Fertilizers, 5, 28-30, 2000.
Read_full_article: pdf/21-2011/21-4-2011/SU21-4-2011-Halasz.pdf
Correspondence: Judit L. Halász. College of Nyíregyháza, Institute of Biology, Nyíregyháza, Nyíregyháza-4400, Sóstói út 31/b. Hungary, email: halaszj@nyf.hu

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Article Title: Effect of pollution in the flora , microflora and soil enzyme activities near to the Upper-Tisza
Authors: Judit L. Halász1*, Zsolt Kotroczo1, Erzsebet Krausz2, Márta D. Tóth1, Sándor Balázsy1
Affiliation: ¹ Institute of Biology, College of Nyíregyháza, Nyíregyháza, Hungary
2Department of Environmental Science, College of Nyíregyháza, Nyíregyháza, Hungary
Abstract: Our main purpose was to study the flora, the microflora and the enzyme activity in soil of polluted areas, and to identify the factors affecting them. Besides studying the percentage distribution of species in the soil, we determined the total number of culturable microbes as well. Enzyme activities of phosphatase, invertase, dehydrogenase, and cellulase, were also measured in the samples of the contaminated soils. Total heavy metal content in soil samples was determined by analysis of Cd, Cu, Fe, Pb and Zn using XRF technique. According to our examinations, ratio of Actinomyces and Pseudomonas genera decreased while the Bacillus sp. was found to be increased in the soils. Wastes reduce the activity of soil enzymes (cellulase, phosphatase, invertase), however, the activity of dehydrogenase increase.
Keywords: enzyme activity, soil microorganisms, plants, contaminated soil
References: Bartholomev JW, Mittwer T, A simlified bacterial spore stain. Stain Tech, 25, 153-154, 1950.
Borie F, Fuenteacba R, Biochemistry of volcanic ash soils:2. Urease activity. Agricultura-Technica, 42, 135-142, 1982.
Chander K, Brookes P, Microbial biomass dynamics during the decomposition of glucose and maize in metal-contaminated and not-contaminated soils. Soil Biology and Biochemistry (Oxford), 23, 917- 925, 1991.
Dick, WA, Tabatabai MA, Significance and potential uses of soil enzymes. In: Blain, F.J. (Ed.), Soil Microbial Ecology Application in Agricultural and Environmental Management. New York: Marcel Dekker, 95-127, 1992.
Frankenberger WT, Johanson JB, Method of measuring invertase activity in soils. Plant and Soil, 74, 301- 311, 1983.
Garcia C, Hernandez T, Costa F, Potential use of dehydrogenase activity as an index of microbial activity in degraded soils. Communications in soil science and plant analysis, 28, 123-134, 1997.
Hattori H, Influence of cadmium on decomposition of glucose and cellulose in soil. Soil Science and Plant Nutrition (Tokyo), 37, 39-45, 1991.
Hucker GJ, Comparison of various methods of Gram staining. Abstr. Bact, 6, 2-8, 1922.
Hugh R, Leifson FI, The taxonomic significance of fermentative versus oxidative metabolism of carbohydrate, by various Gram negative bacteria. Journal of Bacteriologie, 66, 21-28, 1953.
Kovács G, Catalase Activity- an Indicator of Biological Activity in Forest Soil. Agrokémia és Talajtan, 39, 439-443, 1990.
Krámer M, Erdei G, Application of the method of phosphatase activity determination in agricultural chemistry. Soviet Soil Sci, 9, 1100-1103, 1959.
Kuprevics VF, Scserbakova TA, Csjupa GP, Soil urease activity. Dokl. Akad. Navuk. Belarusk, SSR 10, 336-338, 1966.
Lee IS, Kim OK, Chang YY, Bae B, Kim HH, Baek HK, Heavy metal concentracions and enzyme activities in soil from a contaminated Korean shooting range. Journal of Bioscience and Bioengineering, 94, 406- 411, 2002.
Mersi von W, Scinner F, An improved and accurate method for determining the dehydrogenase activity of soils with iodonitrotetrazolium chlorid. Biology and Fertility of Soils, 11, 216-220. 1991.
Moller H, Saccharase and urease content of peat and muick soils in north-west German aldercommunities, 9, 175-192, 1979.
Pascual JA, Moreno JL, Hernández T, Garcia C, Persistence of immobilised and total urease and phosphatase activities in a soil amended with organic wastes. Bioresource Technology, 82, 73-78, 2002.
Pascual I, Antolín MC, Garcia C, Polo A, Díaz MS, Effect of water deficit on microbial characteristics in soil amended with sewage sludge or inorganic fertilizer under laboratory conditions. Bioresource Technology, 98, 29-37, 2007.
Poll C, Thiede A, Wermbter N, Sessitsch A, Kandeler E, Micro-scale distribution of microorganisms and microbial enzyme activities in a soil with longterm organic amendment. European journal of soil science, 54, 715-724, 2003.
Reddy RU, Reddy MS, Jayakumar GWL, Urease activity in soils of northern Telanguna of Andhra pradesh, and its relationship with various soil properties. Annuals of Agricultural Research, 17, 261-264, 1996.
Smith LJ, Papendick RI, Soil organic matter dynamics and crop residue management. In: Metting, B. (Ed9, Soil Microbial Ecology. Marcell Dekker, New York, USA. 1993.
Tabatabai MA, Soil enzymes. In: Micelson, S. H., Bigham, J. E. (Eds.), Methods of Soil Analysis. Part 2: Microbial and Biochemical Properties. Madison: Soil Science Society of America, 775-826, 1994.
Thirukkumaran CM, Parkinson D, Microbial respiration, biomass, metabolic quotient and litter decomposition in a lodgepole pine forest floor amended with nitrogen and phosphorus fertilizer. Soil Biology and Biochemistry, 32, 59-66, 2000.
Unger-féle cellulózteszt módszer a talajbiológiai aktivitás meghatározására. In: Szegi. Talajmikrobiológiai vizsgálati módszerek. 245-247. Mezőgazdasági Kiadó. Budapest. 1979.
Zhang CB, Zhang ZH, A study of dynamic relationship between soil organic matter and enzymatic activity. Soils and Fertilizers, 5, 28-30, 2000.
*Correspondence: Judit L. Halász. College of Nyíregyháza, Institute of Biology, Nyíregyháza, Nyíregyháza-4400, Sóstói út 31/b. Hungary, email: halaszj@nyf.hu