Initiation of Begonia semperflorens cv. ‚Ambassador’ white vitroculture from plant apex of zygote origin

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Article Title:	Initiation of Begonia semperflorens cv. ‚Ambassador’ white vitroculture from plant apex of zygote origin
Authors:	Julieta – Emilia Gergely1*, Dorina Cachiţă-Cosma2
Affiliation:	1 University of Oradea, Faculty of Science, Department of Biology. Oradea. Romania 2 “Vasile Goldiş” Western University of Arad. Arad. Romania
Abstract:	The initiation of Begonia semperflorens cv. ‘Ambassador’ White vitroculture was made by in vitro cultivation of some explants consisting in apical minicuttings, approx. 3-4 mm size, taken from 30 days old seedlings, derived from germinated seeds in aseptic Petri capsules, on filter paper moistened with sterile water. Explants were inoculated and grown – for 30 days – on Murashige-Skoog mineral medium (1962), modified, with added vitamins, sucrose, agar-agar, with or without growth regulators, namely the addition of indolebutyric acid (IBA) and/ or with thidiazuron (TDZ) 0.5 mg/l each. The minicuttings consisted of upper side of hypocotile, which held the bud strain of plant, the two epigeal cotyledons being inserted into its basal zone. After 30 days in vitro culture, the best results in terms of phylogenesis were obtained from the mineral basic medium culture with the addition of 0.5 mg / l TDZ, the number of regenerated leaves was much higher than that recorded on the other experimental variants. But the most balanced regeneration on buds, leaves respectively, as well as on roots was obtained from mineral basal medium culture supplemented with 0.5 mg / l TDZ in combination with 0.5 mg / AIB. If the culture was present 2.5mg /l.4-dichlorophenoxyacetic acid (2,4-D), the morphogenesis consisted only in calusogenesis.
Keywords:	Begonia semperflorens cv. ’Ambassador’ White, cytokine, auxine, micropropagation
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*Correspondence:	Julieta – Emilia Gergely, University of Oradea, Faculty of Science, Department of Biology, 1 Universităţii Str., zip code: 410087, Oradea, Romania, phone: 0040259408448, e-mail: jromocea@uoradea.ro