Flow cytrometric analysis of red blood cells in Polycythemia vera

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Article Title:	Flow cytrometric analysis of red blood cells in Polycythemia vera
Authors:	Ana-Maria Gheorghe1, Alexandrina Rugina1, Andreea Delia Moicean2, Aurel Ardelean3, Daniela Bratosin1,3*
Affiliation:	1National Institute of Biological Science Research and Development (INCDSB), Bucharest, Romania 2Clinical Institute Fundeni, Center of Hematology & Bone Marrow Transplantation ’’St. Berceanu’’, Bucharest 3 ″Vasile Goldis″ Western University of Arad, Faculty of Natural Sciences, Arad, Romania
Abstract:	Polycythemia vera (PV) is characterized by an absolute increase in the red blood cell mass, but the mechanisms are not completely understood. In this study, we identified by flow cytometric analysis morphological forms that deviate from the classical discoid shape, who had a more viability determined by Calcein-AM method and a normal phosphatidylserine exposure level. Measurement of glycoconjugate sialylation using lectines demonstrates a low degree of sialilation of membrane glycoconjugates of Polycythemia vera RBCs but which is close to normal after treatment and an increased percentage of cells with active caspase-8 and -3 compared to normal RBCs, showing that the organism tries to restore the apoptotic mechanism for maintaining the normal hematocrit. Our observations may contribute for understanding the survival of RBCs in Polycythemia vera and may also participate in elucidation of the mechanism in pathogenesis of this disease.
Keywords:	polycythemia vera; PV; rbcs, red blood cells; erythrocyte viability; phosphatidylserine exposure; caspase-3; caspase-8; Annexin-V; Calcein-AM; flow cytometry
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*Correspondence:	Daniela Bratosin, National Institute for Biological Science Research and Development, Spl. Independentei, Nº 296, 060031 Bucharest, Romania, Tel/Fax: 40.21.2200881, E-mail: bratosind@yahoo.com