Effects of some plant extracts on various tumor cell lines

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Article Title:	Effects of some plant extracts on various tumor cell lines
Authors:	Maria Czapar 1, Regina Domina 1, Szabolcs Vigh 1, Endre Mathe 1, George Pribac 2, Liana Mos 2, Simona Damian 2, Violeta Turcus 2, Aurelia Covaci 2, Coralia Cotoraci 2, Lucian Paiusan 2
Affiliation:	1 University College of Nyiregyhaza, Hungary, Institute of Agrar and Molecular Research 2 “Vasile Goldis” Western University of Arad, Romania
Abstract:	Fresh vegetables were obtained from local producers. The preparation of the extracts is alike for the most of used plants, because we have the same target: to characterize them with HLPC and GC techniques, and treat our cell lines cultivating them in these extracts’ solutions. We prefer extracts with low content of cell-damaging components, which could arise from used preparation technology. We have worked with many dilutions of the indicated vegetables and fruits we were looking for the dose response effect. We tried at first with the low concentrations of the extracts then we decide to change them with more concentrated, because we couldn’t demonstrate any change looking the growth and the proliferation of the treated cells. The aim of our investigations is to identify with bioanalytical and biochemical tools the qualitative and quantitative composition of our extracts, and using the DNA microarray technique we would like to show the effect of the vegetal extracts on transcription of the whole genome. The last years we created the possibility of real time imaging microscopy, we can directly follow the changes in life processes of cells induced by vegetal extracts, especially those of cell cycle regulation. Where we want to get? We want direct results of chemopreventive effects of bioactive substances from vegetal extracts, and use these results in functional foods developing.
Keywords:	leafy vegetable, human nutrition, quality, chemical
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*Correspondence:	Maria Czapar, University College of Nyiregyhaza, Hungary, E-mail: marika.czapar@zeus.nyf.hu