Preliminary studies regarding an early detection method applied for malignant melanoma on mice

This is an automatically generated default intro template – please do not edit.

	Read full article
Article Title:	Preliminary studies regarding an early detection method applied for malignant melanoma on mice
Authors:	Corina Tiulea1, Camelia Peev1, Terezia Nyari2, Radu Banica2, Claudia Toma3, Anca Muresan4, Cristina A. Dehelean1
Affiliation:	1 University of Medicine and Pharmacy Victor Babes Timisoara, Romania Faculty of Pharmacy 2 National Institute for Research and Development in Electrochemistry and Condensed Matter, Timisoara, Romania 3 “Vasile Goldis” Western University of Arad, Romania, Faculty of Medicine, Pharmacy and Dental Medicine 4 University of Medicine and Pharmacy Victor Babes Timisoara, Romania, Faculty of Medicine
Abstract:	One of the most devastating types of cancer is represented by cutaneous melanoma. In order to study this pathology models have been developed that recapitulate the condition of melanoma invasion in vivo. In this study B16 mouse model for melanoma has been used on C57BL/6J mice. This work reports a comparative study on cutaneous melanoma evolution investigated by histopathology and UV-Vis-NIR spectroscopy. After housing for four weeks, four C57BL6 female, mice were inoculated with 0.5 ml B16 melanoma cell suspension prepared in the moment of using with saline solution. After three weeks mice with most representative skin lesions were chosen for analyses. Tissue samples (skin) were fixed in 10% formalin solution and were embedded in paraffin and cut at 4 microns. Finally after deparaffinized the samples were stained with H&E (hematoxylin-eosin) and microscopically analyzed. The biopsy for sample 1 was obtained in the day 7 after inoculation and for sample 10 after 20 days. UV-Vis-NIR absorption spectroscopy analyze were also made with a Lambda 950 UV-Vis-NIR double-beam spectrometer (Perkin Elmer) by transmission method. After 3 weeks from the inoculation phase the damages on the skin level are obvious. B16 cells are invasive and determine a metastatic process. In the NIR wavelength range 350-800 nm it can also be observed the evolution of melanoma. The conclusion of this study is that even if spectral data are not enough to make a diagnosis on skin pathology, their qualitative agreement with the histological evaluation results indicate that the method is promising for the development of techniques for the study and characterization of pigmented skin lesions. Further research is needed for the improvement of sample preparation method and development of UV-Vis-NIR diffuse reflectance spectroscopy techniques for this purpose.
Keywords:	cutaneous melanoma, mouse, histopathology, spectroscopy
References:	Okot N., Menashe B.E.- Transcriptional regulation of metastasis-related genes in human melanoma, Clinical & Experimental Metastasis, 20, 251–263, 2003. Gaggioli C., Sahai E.- Melanoma invasion –Current knowledge and future directions, Pigment Cell Res(Review), 20, 161–172, 2007. Bar-Eli M. – Molecular mechanisms of melanoma metastasis, J Cell Physiol, 173, 275–8, 1997. Chin L. – The genetics of malignant melanoma: lessons from mouse and man, Nat rev cancer, 3, 559-570, 2003. Teicher B.A .-Tumor models in cancer research, in Alvarez E: B16 Murine Melanoma, Humana press inc, New Jersey, 73-87, 2002. Yoshiura K., Nishishita T., Nakaoka T., Yamashita N. – Inhibition of B16 melanoma growth and metastasis in C57BL mice by vaccination with a syngeneic endothelial cell line, Journal of Experimental & Clinical Cancer Research, 28,13-28, 2009. Alaluf S., Atkins D., Barrett B., Blount M., Carter N., Heath A. – The impact of epidermal melanin on objective measurements of human skin color, Pigment Cell Res., 15, 119–126, 2002. Kollias N., Stamatas G.N. – Optical non-invasive approaches to diagnosis of skin diseases, J. of Investigation in Dermatology, 7,64–75, 2002. Zhiwei H., Haishan Z., Iltefat H., Abdulmajeed A., Tan E., McLean D., Lui H. – Cutaneous melanin exhibiting fluorescence emission under near-infrared light excitation, Journal of Biomedical Optics, 11(3), 034010 1-6, 2006. Zonios G., Dimou A., Bassukas I., Galaris D., Tsolakidis A., Kaxiras E., -Melanin absorption spectroscopy: new method for noninvasive skin investigation and melanoma detection, Journal of Biomedical Optics, 13(1), 014017 3-8, 2008. Liu Y., Kempf V., Nofsinger J.B., Weinert E.E., Rudnicki M., Wakamatsu K., Ito S, Simon J.D. -“Comparison of the structural and physical properties of human hair eumelanin following enzymatic or acid/base extraction” Pigment Cell Research, 16, 355–365, 2003. Elenitsas R., Schuchter L.M. -The role of the pathologist in the diagnosis of melanoma, Current Opinion in oncology, 10,162-169, 1998. Nickoloff B.J. – Melanoma techniques and protocols, in Slominki A, Carlson A, Wortsman J, Mihn M: Role of molecular biology in diagnostic pathology of melanoma, Humana Press Inc., Totowa, New Jersey, 123-163, 2001. Roomi M.W., Kalinovsky T., Roomi N.W., Ivanov V., Rath M., Niedzwiecki A. – Suppression of growth and hepatic metastasis of murine B16FO melanoma cells by a novel nutrient mixture, Oncology reports 2008, 20, 809-817.
*Correspondence:	Corina Tiulea, University of Medicine and Pharmacy Victor Babes Timisoara, Faculty of Pharmacy, Eftimie Murgu square no.2, 300041,+40744648855; Email:corina_tiulea@yahoo.com