The immunohistochemical detection of substance P (SP) in the pancreas and intestine of three…

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Article Title:	The immunohistochemical detection of substance P (SP) in the pancreas and intestine of three species of lower vertebrates; light-microscopic observations
Authors:	Ioana Trandaburu1, Tiberiu Trandaburu2
Affiliation:	1 Centre of Cytobiology, Institute of Biology, Bucharest, Romania 2 Laboratory of Histology and Embriology, Faculty of Sciences, University of Pitesti, Romania
Abstract:	The pancreas and gut of two amphibians and a reptile species were investigated immunohistochemically for the occurrence and topographic distribution of substance P (SP) by using peroxidase anti-peroxidase (PAP) procedure. A relative rich SP innervation consisting of nerve fibbers with a peri-acinar distribution was seen both in frogs and turtle pancreas. In turtles, thick bundles of nerve fibbers, localized in the pancreatic connective tissue, have been also observed. In addition to the neural structures, the pancreas of frogs and turtles displayed often singular immunostained cells of endocrine nature. Finally, both varieties of “closed” and “open” enteroendocrine cells have been immunodetected in the villi epithelium of these animals. The above findings are discussed in connection with those previously reported in other taxa of vertebrates.
Keywords:	substance P (SP), amphibians, turtle, pancreas, intestine, immunohistochemistry
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*Correspondence:	Ioana Trandaburu, Institute of Biology, Spl. Independenţei 296, 060031, Bucharest, e-mail : itrandaburu@yahoo.com; ioana.trandaburu@ibiol.ro