Estimation of genotoxic potential of carbendazim in fenugreek

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Article Title:	Estimation of genotoxic potential of carbendazim in fenugreek
Authors:	Elena Truta1*, Gabriela Capraru1, Maria Magdalena Zamfirache2, Maria Asaftei3, Constantin Toma2, Zenovia Olteanu2, Lacramioara Ivanescu2
Affiliation:	1 Biological Research Institute of Iasi 2 “Al. I. Cuza” University of Iaşi, Faculty of Biology 3 Clinical Hospital “I.C. Parhon” of Iaşi
Abstract:	Because the studies on pesticide effects evidenced a diversity of chromosomal aberrations, in relation to the analysed class of compounds, we considered necessary to test the influence of carbendazim fungicide on genetic material of fenugreek (Trigonella foenum graecum L.), which can constitute, as other plant species, monitoring systems in the evaluation of possible genetic risk of the pesticide use. Mitotic index, the frequency of cell division phases, the types and incidence of ana-telophase aberrations, and the types of metaphase abnormalities were studied. The mitotic index was lower in all carbendazim treated variants; the most numerous ana-telophase aberrations and metaphase abnormalities were noted in 0.5% and 1.0% carbendazim treated variants. The effect of carbendazim on height growth of plantlets in early ontogenetic phases was also investigated – an inverse relation between concentration and plantlets’ height was registered.
Keywords:	carbendazim, chromosome aberrations, mitotic index, Trigonella foenum graecum L.
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*Correspondence:	Elena Truta, Department of Cell Biology, Biological Research Institute, Lascar Catargiu nr. 47, 700107 Iasi, Romania, email: trutaelena@yahoo.com