Oxidative stress in Diabetes mellitus type 2 complicated with nephropathy

Authors: Daniela MATEI¹*, Corina MOLDOVAN¹, Mariana MUREŞAN¹, Otilia MICLE¹, Liana MICLE¹,

Ovidiu BURTĂ¹, Adriana PIRTE¹, Adriana MUREŞAN²

Affiliation: ¹ Faculty of Medicine and Pharmacy, University of Oradea, Romania; ² University of Medicine and Pharmacy I. Hatieganu, Cluj- Napoca, Romania

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ABSTRACT. Increase formation of reactive oxygen species (ROS) induced by hyperglycaemia has been suggested to contribute to the development of diabetes mellitus complications, including nephropathy. The purpose of this study was to test the hypothesis that in diabetic nephropathy from diabetes mellitus type 2 there was oxidative stress. We quantified the level of oxidative stress by measuring the concentration of malondialdehyde, carbonylated proteins and caeruloplasmin in five groups of subjects suffering from diabetes mellitus type 2 and diabetic nephropathy. We obtained a significant increased level of malondialdehyde and carbonylated proteins in study groups by comparison with the control group (p< 0,001) and a decrease of caeruloplasmin concentration. These results demonstrated that there was oxidative stress in diabetes mellitus type 2 with complicated with nephropathy and its level increased with the stage of diabetic nephropathy. Oxidative stress could be a generating factor of the complications of diabetes mellitus, including nephropathy.

Keywords: diabetic nephropathy, oxidative stress, malondialdehyde, carbonylated proteins caeruloplasmin