Growth Inhibition And Oxidative Stress In The Freshwater Ciliate Paramecium Sp Exposed To Copper Oxide Nanoparticles Print
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Article Title: Growth Inhibition And Oxidative Stress In The Freshwater Ciliate Paramecium Sp Exposed To Copper Oxide Nanoparticles
Authors: Bouraiou C., Berrebbah H., Djekoun M., Bouarroudj T., Yahyaoui A., Khene L., Kahli H., Djebar M. R
Affiliation: 1 Laboratory of Cellular Toxicology. Department of Biology. Faculty of Science, Badji Mokhtar University, Annaba, Algeria 2 Laboratory of Chemistry Inorganic Materials (LCMI), Department of Chemistry, Faculty of Science, Badji Mokhtar University, Annaba, Algeria
Abstract: Copper oxide nanoparticles (CuO-NPs) are widely used in various industrial and commercial applications, but little is known about their potential hazard in freshwater ciliate. In this study we investigated the effects of CuO-NPs in an alternative model Paramecium sp. which is perfectly adapted with toxicological studies. Paramecium cells were exposed in triplicate to control, 30, 60, 90 and 120 mg CuO L-1, and the growth kinetics was followed for 120 hours, enzymatic activities of Superoxide Dismutase (SOD), Catalase (CAT), Glutathione-S-Transferase (GST) and Glutathione content (GSH) were investigated to evaluate antioxidative response to copper oxide nanoparticles. Our results showed a dose-dependent inhibition of cell growth, on another hand, the monitoring of biomarkers revealed induction of SOD, CAT, and GST activities, in parallel a significant decrease on GSH content compared to control.
Keywords: Paramecium sp, growth kinetic, oxidative stress, CuO, toxicity
*Correspondence: Chiraz Bouraiou, Laboratory of Cellular Toxicology. Department of Biology. Faculty of Science, Badji Mokhtar University, Annaba, Algeria, This e-mail address is being protected from spambots. You need JavaScript enabled to view it